Q8A806 (MURB_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BT_1368

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224664
Regions
	Domain	12 – 181	170	FAD-binding PCMH-type
Sites
	Active site	157	1	By similarity
	Active site	229	1	Proton donor By similarity
	Active site	325	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A806 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F8462F9FA75AB0CC	FASTA	331	36,834
	10 20 30 40 50 60 MYSLLSHNTF GIDVYAERFQ EYASVEELKT LIAQGALTTP FLHIGGGSNL LFVKDYEGLV 70 80 90 100 110 120 LHSRIEGIEV TEEDERSVAV RVGAGVVWDD FVGYCVEHGW YGTENLSLIP GEVGASAVQN 130 140 150 160 170 180 IGAYGVEVKD LITSVETVNI HGEERVYAVD ECGYAYRDSI FKRPENKSVF VTYVCFRLSK 190 200 210 220 230 240 EERYTLDYGT IRQELEKYPE LTLPILRKVI IDIRESKLPD PKVMGNAGSF FMNPIVPREK 250 260 270 280 290 300 LEALQQEFPR IPFYELNDGR VKIPAGWMID QCGWKGKALG PAAVHDKQAL VLVNRGGAKG 310 320 330 SDVLALSDAV RASVRAKFGI DIHPEVNLVG K

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO76475.1.
RefSeq	NP_810281.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A806.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1073012.
GenomeReviews	Gene locus BT_1368 in contig AE015928_GR.
KEGG	bth:BT_1368.
NMPDR	fig|226186.1.peg.1368.
PATRIC	21057603. VBIBacThe70966_1403.
Phylogenomic databases
HOGENOM	HBG686573.
OMA	IEQAGWK.
PhylomeDB	Q8A806.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_1368-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BACTN

Accession

Primary (citable) accession number: Q8A806

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program