Reviewed, UniProtKB/Swiss-Prot Q8A7Z7 (HIS2_BACTN)

Names and origin

Protein names

Recommended name:
    Histidine biosynthesis bifunctional protein hisIE
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosyl-AMP cyclohydrolase
                Short name=PRA-CH
              EC=3.5.4.19
    2- Recommended name:
            Phosphoribosyl-ATP pyrophosphatase
                Short name=PRA-PH
              EC=3.6.1.31

Gene names

Name:	hisI
Synonyms:	hisIE
Ordered Locus Names:	BT_1377

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-ATP + H2O = 1-(5-phosphoribosyl)-AMP + diphosphate. HAMAP MF_01019 1-(5-phosphoribosyl)-AMP + H2O = 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide. HAMAP MF_01019
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9. HAMAP MF_01019 Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.
Subcellular location	Cytoplasm By similarity HAMAP MF_01019.
Sequence similarities	In the N-terminal section; belongs to the PRA-CH family. In the C-terminal section; belongs to the PRA-PH family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosyl-AMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosyl-ATP diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Histidine biosynthesis bifunctional protein hisIE HAMAP MF_01019		PRO_0000136404
Regions
	Region	1 – 108	108	Phosphoribosyl-AMP cyclohydrolase HAMAP MF_01019
	Region	109 – 203	95	Phosphoribosyl-ATP pyrophosphohydrolase HAMAP MF_01019

Sequences

	Sequence		Length	Mass (Da)
	Q8A7Z7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 451226871EA0A2A2	FASTA	203	23,078
	10 20 30 40 50 60 MELDFDKMNG LVPAIIQDNE TRKVLMLGFM NKEAYDKTVE TGKVTFFSRT KNRLWTKGEE 70 80 90 100 110 120 SGNFLHVVSI KADCDNDTLL IQADPAGPVC HTGTDTCWGE KNEEPVMFLK ALQDFIDKRH 130 140 150 160 170 180 EEMPQGSYTT SLFESGINKI AQKVGEEAVE TVIEATNGTN ERLIYEGADL IYHMIVLLTS 190 200 KGYRIEDLAR ELQERHSSTW KKH

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO76484.1.
RefSeq	NP_810290.1.
3D structure databases
SMR	Q8A7Z7. Positions 1-101, 108-197.
ModBase	Search...
Genome annotation databases
GeneID	1072959.
GenomeReviews	Gene locus BT_1377 in contig AE015928_GR.
KEGG	bth:BT_1377.
NMPDR	fig|226186.1.peg.1377.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG294308.
OMA	VHYWSRS.
PhylomeDB	Q8A7Z7.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_1377-MONOMER.
BRENDA	3.5.4.19. 21018. 3.6.1.31. 21018.
Family and domain databases
HAMAP	MF_01019. HisIE. [Tree]
InterPro	IPR002496. PRA_CycHdrlase. IPR008179. PRib-ATP_pyrophosphohydrolase. IPR021130. PRib-ATP_pyroPHydrolase-like. [Graphical view]
Pfam	PF01502. PRA-CH. 1 hit. PF01503. PRA-PH. 1 hit. [Graphical view]
ProDom	PD002610. PRA_CycHdrlase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03188. histidine_hisI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS2_BACTN

Accession

Primary (citable) accession number: Q8A7Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)