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Q8A7V7 (NRFA_BACTN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome c-552
EC=1.7.2.2
Alternative name(s):
Ammonia-forming cytochrome c nitrite reductase
Short name=Cytochrome c nitrite reductase
Gene names
Name:nrfA
Ordered Locus Names:BT_1417
OrganismBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifier226186 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity. HAMAP MF_01182

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity. HAMAP MF_01182

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity HAMAP MF_01182.

Miscellaneous

This protein is not predicted to have a signal sequence by the program SignalP. All others members of this family are periplasmic proteins. HAMAP MF_01182

Sequence similarities

Belongs to the cytochrome c-552 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 493468Cytochrome c-552 HAMAP MF_01182
PRO_0000268960

Sites

Metal binding1161Iron (heme 3 axial ligand) By similarity
Metal binding1481Iron (heme 1 axial ligand) By similarity
Metal binding1861Iron (heme 2 axial ligand) By similarity
Metal binding2281Iron (heme 3 axial ligand) By similarity
Metal binding2301Calcium By similarity
Metal binding2311Calcium; via carbonyl oxygen By similarity
Metal binding2761Calcium; via carbonyl oxygen By similarity
Metal binding2781Calcium By similarity
Metal binding2901Iron (heme 5 axial ligand) By similarity
Metal binding3011Iron (heme 4 axial ligand) By similarity
Metal binding3151Iron (heme 2 axial ligand) By similarity
Metal binding3321Iron (heme 5 axial ligand) By similarity
Metal binding4071Iron (heme 4 axial ligand) By similarity
Binding site1441Heme 1 (covalent) By similarity
Binding site1471Heme 1 (covalent) By similarity
Binding site1821Heme 2 (covalent) By similarity
Binding site1851Heme 2 (covalent) By similarity
Binding site2241Heme 3 (covalent) By similarity
Binding site2271Heme 3 (covalent) By similarity
Binding site2311Substrate By similarity
Binding site2791Substrate By similarity
Binding site2971Heme 4 (covalent) By similarity
Binding site3001Heme 4 (covalent) By similarity
Binding site3281Heme 5 (covalent) By similarity
Binding site3311Heme 5 (covalent) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8A7V7 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: BDF779458684229C

FASTA49355,910
        10         20         30         40         50         60 
MEKKLKSWQG WLLSGGSMVV VFVLGLCVSA LMERRAEVAS IFNNRKTVIK GIEARNELFK 

        70         80         90        100        110        120 
DDFPREYQTW TETAKTDFES EFNGNVAVDA LEKRPEMVIL WAGYAFSKDY STPRGHMHAI 

       130        140        150        160        170        180 
EDITASLRTG SPAGPHDGPQ PSTCWTCKSP DVPRMMEALG VDSFYNNKWA AFGDEIVNPI 

       190        200        210        220        230        240 
GCSDCHDPET MNLHISRPAL IEAFQRQGKD ITKATPQEMR SLVCAQCHVE YYFKGDGKYL 

       250        260        270        280        290        300 
TFPWDKGFTV EDMEAYYDEA GFYDYIHKLS RTPILKAQHP DYEIAQMGIH GQRGVSCADC 

       310        320        330        340        350        360 
HMPYKSEGGV KFSDHHIQSP LAMIDRTCQT CHRESEETLR NNVYERQRKA NEIRNRLEQE 

       370        380        390        400        410        420 
LAKAHIEAKF AWDKGATEDQ MKDVLALIRQ AQWRWDFGVA SHGGSFHAPQ EIQRILSHGL 

       430        440        450        460        470        480 
DRAMQARLAV SKVLAKHGYT EDVPMPDIST KAKAQEYIGL DMDAERAAKE KFLKTTVPAW 

       490 
LEKAKANGRL AQK

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References

[1]"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE015928 Genomic DNA. Translation: AAO76524.1.
RefSeqNP_810330.1. NC_004663.1.

3D structure databases

HSSPHSSP built from PDB template 1FS7 based on UniProtKB Q9S1E5.
ProteinModelPortalQ8A7V7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1071634.
GenomeReviewsGene locus BT_1417 in contig AE015928_GR.
KEGGbth:BT_1417.
NMPDRfig|226186.1.peg.1417.
PATRIC21057701. VBIBacThe70966_1450.

Phylogenomic databases

HOGENOMHBG488281.
OMAPFAKASK.
PhylomeDBQ8A7V7.
ProtClustDBPRK11125.

Enzyme and pathway databases

BioCycBTHE226186:BT_1417-MONOMER.

Family and domain databases

HAMAPMF_01182. Cytochrom_C552.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
KOK03385.
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNRFA_BACTN
AccessionPrimary (citable) accession number: Q8A7V7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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