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Protein

Biotin biosynthesis bifunctional protein BioAB

Gene

bioB

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes two activities which are involved in the biotine biosynthesis: the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism, and the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA).By similarity

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.By similarity
  • pyridoxal 5'-phosphateBy similarity

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin biosynthesis bifunctional protein BioAB (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Pathwayi: biotin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route).
Proteins known to be involved in this subpathway in this organism are:
  1. Biotin biosynthesis bifunctional protein BioAB (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route), the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi66Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi69Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi106Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi138Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi198Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi268Iron-sulfur 2 (2Fe-2S)By similarity1
Sitei333Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMBy similarity1
Binding sitei3687-keto-8-aminopelargonic acidBy similarity1
Binding sitei4617-keto-8-aminopelargonic acidBy similarity1
Binding sitei562Pyridoxal phosphateBy similarity1
Binding sitei5917-keto-8-aminopelargonic acidBy similarity1
Binding sitei6247-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity1
Binding sitei7087-keto-8-aminopelargonic acidBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Multifunctional enzyme, Transferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00160
UPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin biosynthesis bifunctional protein BioAB
Including the following 2 domains:
Biotin synthase BioB (EC:2.8.1.6)
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA (EC:2.6.1.62)
Alternative name(s):
7,8-diamino-pelargonic acid aminotransferase
Short name:
DAPA AT
Short name:
DAPA aminotransferase
7,8-diaminononanoate synthase
Short name:
DANS
Diaminopelargonic acid synthase
Gene namesi
Name:bioB
Ordered Locus Names:BT_1442
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003812341 – 741Biotin biosynthesis bifunctional protein BioABAdd BLAST741

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei591N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ8A7T2
PRIDEiQ8A7T2

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi226186.BT_1442

Structurei

3D structure databases

ProteinModelPortaliQ8A7T2
SMRiQ8A7T2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni428 – 429Pyridoxal phosphate binding2
Regioni625 – 626Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

In the N-terminal section; belongs to the radical SAM superfamily. Biotin synthase family.Curated
In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C8Y Bacteria
COG0161 LUCA
COG0502 LUCA
HOGENOMiHOG000138865
InParanoidiQ8A7T2
KOiK00833
OMAiKWCAQSS
OrthoDBiPOG091H01IB

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.20.20.70, 1 hit
3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_00834 BioA, 1 hit
MF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR005814 Aminotrans_3
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR005815 BioA
IPR002684 Biotin_synth/BioAB
IPR006638 Elp3/MiaB/NifB
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
IPR007197 rSAM
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00508 bioA, 1 hit
TIGR00433 bioB, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q8A7T2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQEIKDQV LAGFDISSAQ ATWLANMADS EALYAAAHEI TITCASHEFD
60 70 80 90 100
MCSIINAKSG RCPENCKWCA QSSHYKTQAE IYDLLPAEEC LRQAKYNESQ
110 120 130 140 150
DVNRFSLVTS GRKPSPKQIS QLCDAARLMR KHSSIQLCAS LGLLNEEELR
160 170 180 190 200
ALHTAGITRY HCNLETAPSY FPTLCSTHTQ EQKLATLDAA RRVGMDICCG
210 220 230 240 250
GIIGMGETME QRIEFAFTLA ELNVQSIPIN LLSPIPGTPL ENEKALSEEE
260 270 280 290 300
ILRTIALFRF INPTAFLRFA GGRSQLTPEA MRKALFVGIN SAIVGDLLTT
310 320 330 340 350
LGSKVSDDKK MILEEGYHFA DSQFDREHLW HPYTSTTDPL PVYKVKRADG
360 370 380 390 400
ATITLEDGRT LIEGMSSWWC AVHGYNHPVL NQAAKDQLDK MSHVMFGGLT
410 420 430 440 450
HDPAIELGKL LLPLVPPSMQ KIFYADSGSV AVEVALKMAV QYWYAAGKPD
460 470 480 490 500
KNNFVTIRSG YHGDTWNAMS VCDPVTGMHS LFGSSLPVRY FVPAPSSRFD
510 520 530 540 550
GEWNPDEIIP LRETIEKHSK ELAALILEPI VQGAGGMWFY HPQYLREAEK
560 570 580 590 600
LCKEHDILLI FDEIATGFGR TGKLFAWEHA GVEPDIMCIG KALTGGYMTL
610 620 630 640 650
SAVLASNQIA DTISNHAPKA FMHGPTFMGN PLACAVACAS VRLLLDSGWA
660 670 680 690 700
ENVKRIEAQL KEELAPARKF PQVADVRILG AIGVIQTERS VSMAYMQRRF
710 720 730 740
VEEGIWVRPF GKLVYLMPPF IISPEQLSKL TSGVLKIVRE M
Length:741
Mass (Da):82,116
Last modified:July 28, 2009 - v2
Checksum:i6FA1F6EE5879C1DD
GO

Sequence cautioni

The sequence AAO76549 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA Translation: AAO76549.1 Different initiation.
RefSeqiNP_810355.1, NC_004663.1

Genome annotation databases

EnsemblBacteriaiAAO76549; AAO76549; BT_1442
GeneIDi1076134
KEGGibth:BT_1442
PATRICifig|226186.12.peg.1474

Similar proteinsi

Entry informationi

Entry nameiBIOAB_BACTN
AccessioniPrimary (citable) accession number: Q8A7T2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 23, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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