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Q8A7T2

- BIOAB_BACTN

UniProt

Q8A7T2 - BIOAB_BACTN

Protein

Biotin biosynthesis bifunctional protein BioAB

Gene

bioB

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes two activities which are involved in the biotine biosynthesis: the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism, and the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA).By similarity

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.
    S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.By similarity
    Pyridoxal phosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi106 – 1061Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S)By similarity
    Metal bindingi268 – 2681Iron-sulfur 2 (2Fe-2S)By similarity
    Sitei333 – 3331Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMBy similarity
    Binding sitei368 – 36817-keto-8-aminopelargonic acidBy similarity
    Binding sitei461 – 46117-keto-8-aminopelargonic acidBy similarity
    Binding sitei562 – 5621Pyridoxal phosphateBy similarity
    Binding sitei591 – 59117-keto-8-aminopelargonic acidBy similarity
    Binding sitei624 – 62417-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity
    Binding sitei708 – 70817-keto-8-aminopelargonic acidBy similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: UniProtKB-EC
    4. biotin synthase activity Source: UniProtKB-EC
    5. metal ion binding Source: UniProtKB-KW
    6. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBTHE226186:GJXV-1471-MONOMER.
    UniPathwayiUPA00078; UER00160.
    UPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin biosynthesis bifunctional protein BioAB
    Including the following 2 domains:
    Biotin synthase BioB (EC:2.8.1.6)
    Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA (EC:2.6.1.62)
    Alternative name(s):
    7,8-diamino-pelargonic acid aminotransferase
    Short name:
    DAPA AT
    Short name:
    DAPA aminotransferase
    7,8-diaminononanoate synthase
    Short name:
    DANS
    Diaminopelargonic acid synthase
    Gene namesi
    Name:bioB
    Ordered Locus Names:BT_1442
    OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
    Taxonomic identifieri226186 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000001414: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 741741Biotin biosynthesis bifunctional protein BioABPRO_0000381234Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei591 – 5911N6-(pyridoxal phosphate)lysineBy similarity

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi226186.BT_1442.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8A7T2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni428 – 4292Pyridoxal phosphate binding
    Regioni625 – 6262Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the radical SAM superfamily. Biotin synthase family.Curated
    In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000138865.
    KOiK00833.
    OMAiKWCAQSS.
    OrthoDBiEOG6QVRHN.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    HAMAPiMF_00834. BioA.
    MF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR005814. Aminotrans_3.
    IPR010722. BATS_dom.
    IPR005815. BioA.
    IPR002684. Biotin_synth/BioAB.
    IPR006638. Elp3/MiaB/NifB.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR11986. PTHR11986. 1 hit.
    PfamiPF00202. Aminotran_3. 1 hit.
    PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR00508. bioA. 1 hit.
    TIGR00433. bioB. 1 hit.
    PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8A7T2-1 [UniParc]FASTAAdd to Basket

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    MTLQEIKDQV LAGFDISSAQ ATWLANMADS EALYAAAHEI TITCASHEFD    50
    MCSIINAKSG RCPENCKWCA QSSHYKTQAE IYDLLPAEEC LRQAKYNESQ 100
    DVNRFSLVTS GRKPSPKQIS QLCDAARLMR KHSSIQLCAS LGLLNEEELR 150
    ALHTAGITRY HCNLETAPSY FPTLCSTHTQ EQKLATLDAA RRVGMDICCG 200
    GIIGMGETME QRIEFAFTLA ELNVQSIPIN LLSPIPGTPL ENEKALSEEE 250
    ILRTIALFRF INPTAFLRFA GGRSQLTPEA MRKALFVGIN SAIVGDLLTT 300
    LGSKVSDDKK MILEEGYHFA DSQFDREHLW HPYTSTTDPL PVYKVKRADG 350
    ATITLEDGRT LIEGMSSWWC AVHGYNHPVL NQAAKDQLDK MSHVMFGGLT 400
    HDPAIELGKL LLPLVPPSMQ KIFYADSGSV AVEVALKMAV QYWYAAGKPD 450
    KNNFVTIRSG YHGDTWNAMS VCDPVTGMHS LFGSSLPVRY FVPAPSSRFD 500
    GEWNPDEIIP LRETIEKHSK ELAALILEPI VQGAGGMWFY HPQYLREAEK 550
    LCKEHDILLI FDEIATGFGR TGKLFAWEHA GVEPDIMCIG KALTGGYMTL 600
    SAVLASNQIA DTISNHAPKA FMHGPTFMGN PLACAVACAS VRLLLDSGWA 650
    ENVKRIEAQL KEELAPARKF PQVADVRILG AIGVIQTERS VSMAYMQRRF 700
    VEEGIWVRPF GKLVYLMPPF IISPEQLSKL TSGVLKIVRE M 741
    Length:741
    Mass (Da):82,116
    Last modified:July 28, 2009 - v2
    Checksum:i6FA1F6EE5879C1DD
    GO

    Sequence cautioni

    The sequence AAO76549.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO76549.1. Different initiation.
    RefSeqiNP_810355.1. NC_004663.1.

    Genome annotation databases

    EnsemblBacteriaiAAO76549; AAO76549; BT_1442.
    GeneIDi1076134.
    KEGGibth:BT_1442.
    PATRICi21057749. VBIBacThe70966_1474.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO76549.1 . Different initiation.
    RefSeqi NP_810355.1. NC_004663.1.

    3D structure databases

    ProteinModelPortali Q8A7T2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226186.BT_1442.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO76549 ; AAO76549 ; BT_1442 .
    GeneIDi 1076134.
    KEGGi bth:BT_1442.
    PATRICi 21057749. VBIBacThe70966_1474.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000138865.
    KOi K00833.
    OMAi KWCAQSS.
    OrthoDBi EOG6QVRHN.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00160 .
    UPA00078 ; UER00162 .
    BioCyci BTHE226186:GJXV-1471-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    HAMAPi MF_00834. BioA.
    MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR005814. Aminotrans_3.
    IPR010722. BATS_dom.
    IPR005815. BioA.
    IPR002684. Biotin_synth/BioAB.
    IPR006638. Elp3/MiaB/NifB.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR11986. PTHR11986. 1 hit.
    Pfami PF00202. Aminotran_3. 1 hit.
    PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR00508. bioA. 1 hit.
    TIGR00433. bioB. 1 hit.
    PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
      Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
      Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

    Entry informationi

    Entry nameiBIOAB_BACTN
    AccessioniPrimary (citable) accession number: Q8A7T2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3