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Q8A7T2

- BIOAB_BACTN

UniProt

Q8A7T2 - BIOAB_BACTN

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Protein

Biotin biosynthesis bifunctional protein BioAB

Gene

bioB

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes two activities which are involved in the biotine biosynthesis: the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism, and the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA).By similarity

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.By similarity
  • pyridoxal 5'-phosphateBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity
Metal bindingi106 – 1061Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S)By similarity
Metal bindingi268 – 2681Iron-sulfur 2 (2Fe-2S)By similarity
Sitei333 – 3331Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMBy similarity
Binding sitei368 – 36817-keto-8-aminopelargonic acidBy similarity
Binding sitei461 – 46117-keto-8-aminopelargonic acidBy similarity
Binding sitei562 – 5621Pyridoxal phosphateBy similarity
Binding sitei591 – 59117-keto-8-aminopelargonic acidBy similarity
Binding sitei624 – 62417-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity
Binding sitei708 – 70817-keto-8-aminopelargonic acidBy similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: UniProtKB-EC
  4. biotin synthase activity Source: UniProtKB-EC
  5. metal ion binding Source: UniProtKB-KW
  6. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-1471-MONOMER.
UniPathwayiUPA00078; UER00160.
UPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin biosynthesis bifunctional protein BioAB
Including the following 2 domains:
Biotin synthase BioB (EC:2.8.1.6)
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA (EC:2.6.1.62)
Alternative name(s):
7,8-diamino-pelargonic acid aminotransferase
Short name:
DAPA AT
Short name:
DAPA aminotransferase
7,8-diaminononanoate synthase
Short name:
DANS
Diaminopelargonic acid synthase
Gene namesi
Name:bioB
Ordered Locus Names:BT_1442
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000001414: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741Biotin biosynthesis bifunctional protein BioABPRO_0000381234Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei591 – 5911N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi226186.BT_1442.

Structurei

3D structure databases

ProteinModelPortaliQ8A7T2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni428 – 4292Pyridoxal phosphate binding
Regioni625 – 6262Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the radical SAM superfamily. Biotin synthase family.Curated
In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.Curated

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000138865.
InParanoidiQ8A7T2.
KOiK00833.
OMAiKWCAQSS.
OrthoDBiEOG6QVRHN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00834. BioA.
MF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR005814. Aminotrans_3.
IPR010722. BATS_dom.
IPR005815. BioA.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00508. bioA. 1 hit.
TIGR00433. bioB. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8A7T2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLQEIKDQV LAGFDISSAQ ATWLANMADS EALYAAAHEI TITCASHEFD
60 70 80 90 100
MCSIINAKSG RCPENCKWCA QSSHYKTQAE IYDLLPAEEC LRQAKYNESQ
110 120 130 140 150
DVNRFSLVTS GRKPSPKQIS QLCDAARLMR KHSSIQLCAS LGLLNEEELR
160 170 180 190 200
ALHTAGITRY HCNLETAPSY FPTLCSTHTQ EQKLATLDAA RRVGMDICCG
210 220 230 240 250
GIIGMGETME QRIEFAFTLA ELNVQSIPIN LLSPIPGTPL ENEKALSEEE
260 270 280 290 300
ILRTIALFRF INPTAFLRFA GGRSQLTPEA MRKALFVGIN SAIVGDLLTT
310 320 330 340 350
LGSKVSDDKK MILEEGYHFA DSQFDREHLW HPYTSTTDPL PVYKVKRADG
360 370 380 390 400
ATITLEDGRT LIEGMSSWWC AVHGYNHPVL NQAAKDQLDK MSHVMFGGLT
410 420 430 440 450
HDPAIELGKL LLPLVPPSMQ KIFYADSGSV AVEVALKMAV QYWYAAGKPD
460 470 480 490 500
KNNFVTIRSG YHGDTWNAMS VCDPVTGMHS LFGSSLPVRY FVPAPSSRFD
510 520 530 540 550
GEWNPDEIIP LRETIEKHSK ELAALILEPI VQGAGGMWFY HPQYLREAEK
560 570 580 590 600
LCKEHDILLI FDEIATGFGR TGKLFAWEHA GVEPDIMCIG KALTGGYMTL
610 620 630 640 650
SAVLASNQIA DTISNHAPKA FMHGPTFMGN PLACAVACAS VRLLLDSGWA
660 670 680 690 700
ENVKRIEAQL KEELAPARKF PQVADVRILG AIGVIQTERS VSMAYMQRRF
710 720 730 740
VEEGIWVRPF GKLVYLMPPF IISPEQLSKL TSGVLKIVRE M
Length:741
Mass (Da):82,116
Last modified:July 28, 2009 - v2
Checksum:i6FA1F6EE5879C1DD
GO

Sequence cautioni

The sequence AAO76549.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO76549.1. Different initiation.
RefSeqiNP_810355.1. NC_004663.1.

Genome annotation databases

EnsemblBacteriaiAAO76549; AAO76549; BT_1442.
GeneIDi1076134.
KEGGibth:BT_1442.
PATRICi21057749. VBIBacThe70966_1474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO76549.1 . Different initiation.
RefSeqi NP_810355.1. NC_004663.1.

3D structure databases

ProteinModelPortali Q8A7T2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 226186.BT_1442.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO76549 ; AAO76549 ; BT_1442 .
GeneIDi 1076134.
KEGGi bth:BT_1442.
PATRICi 21057749. VBIBacThe70966_1474.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000138865.
InParanoidi Q8A7T2.
KOi K00833.
OMAi KWCAQSS.
OrthoDBi EOG6QVRHN.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00160 .
UPA00078 ; UER00162 .
BioCyci BTHE226186:GJXV-1471-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPi MF_00834. BioA.
MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR005814. Aminotrans_3.
IPR010722. BATS_dom.
IPR005815. BioA.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR11986. PTHR11986. 1 hit.
Pfami PF00202. Aminotran_3. 1 hit.
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR00508. bioA. 1 hit.
TIGR00433. bioB. 1 hit.
PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
    Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
    Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Entry informationi

Entry nameiBIOAB_BACTN
AccessioniPrimary (citable) accession number: Q8A7T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: November 26, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3