Reviewed, UniProtKB/Swiss-Prot Q8A6N4 (PURA_BACTN)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	BT_1843

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000095147
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	140	1	By similarity
	Active site	147	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A6N4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1569243D88FB2FDB	FASTA	423	46,789
	10 20 30 40 50 60 MKVDVLLGLQ WGDEGKGKVV DVLTPKYDVV ARFQGGPNAG HTLEFEGQKY VLRSIPSGIF 70 80 90 100 110 120 QGDKVNIIGN GVVLDPALFK AEAEALEASG HPLKERLHIS KKAHLILPTH RILDAAYEAA 130 140 150 160 170 180 KGDAKVGTTG KGIGPTYTDK VSRNGVRVGD ILHNFEQKYG AAKARHEQIL KSLNYEYDLT 190 200 210 220 230 240 ELEKAWMEGI EYLKQFHFVD SEHEVNNYLK DGKSVLCEGA QGTMLDIDFG SYPFVTSSNT 250 260 270 280 290 300 VCAGACTGLG VAPNRIGEVF GIFKAYCTRV GAGPFPTELF DETGDKMCTL GHEFGSVTGR 310 320 330 340 350 360 KRRCGWIDLV ALKYSVMING VTKLIMMKSD VLDTFDTIKA CVAYKVDGEE IDYFPYDITE 370 380 390 400 410 420 GVEPVYAELP GWKTDMTKMQ SEDEFPEEFN AYLTFLEEQL GVEIKIVSVG PDRAQTIERY TEE

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO76950.1.
RefSeq	NP_810756.1.
3D structure databases
HSSP	HSSP built from PDB template 1ADE based on UniProtKB P12283.
ModBase	Search...
Genome annotation databases
GeneID	1075166.
GenomeReviews	Gene locus BT_1843 in contig AE015928_GR.
KEGG	bth:BT_1843.
NMPDR	fig|226186.1.peg.1843.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A6N4.
OMA	IPVCVAY.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_1843-MON.
BRENDA	6.3.4.4. 21018.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_BACTN

Accession

Primary (citable) accession number: Q8A6N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)