Q8A684 (DXR_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	BT_2002

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_0000163615
Regions
	Nucleotide binding	15 – 44	30	NADP By similarity
Sites
	Metal binding	156	1	Divalent metal cation By similarity
	Metal binding	158	1	Divalent metal cation By similarity
	Metal binding	227	1	Divalent metal cation By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A684 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 3006AD438940200D	FASTA	390	42,742
	10 20 30 40 50 60 MDSEKKNKKK QIAILGSTGS IGTQALQVIE EHPDLYEAYA LTANNRVELL IAQARKFQPE 70 80 90 100 110 120 VVVIANEEKY AQLKEALSDL PIKVYAGIDA VCQIVEAGPV DMVLTAMVGY AGLKPTINAI 130 140 150 160 170 180 RAKKAIALAN KETLVVAGEL INQLAQQYHT PILPVDSEHS AVFQCLAGEV GNPIEKVILT 190 200 210 220 230 240 ASGGPFRTCT LEQLKSVTKT QALKHPNWEM GAKITIDSAS MMNKGFEVIE AKWLFGVQPS 250 260 270 280 290 300 QIEVVVHPQS VIHSMVQFED GAVKAQLGMP DMRLPIQYAF SYPDRICSSF DRLDFTQCTN 310 320 330 340 350 360 LTFEQPDTKR FRNLALAYEA MYRGGNMPCI VNAANEVVVA AFLRDGISFL GMSDVIEKTM 370 380 390 ERAAFVAAPA YDDYVATDAE ARRIAAELIP

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO77109.1.
RefSeq	NP_810915.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A684.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1076106.
GenomeReviews	Gene locus BT_2002 in contig AE015928_GR.
KEGG	bth:BT_2002.
NMPDR	fig|226186.1.peg.2002.
PATRIC	21058929. VBIBacThe70966_2053.
Phylogenomic databases
HOGENOM	HBG430762.
OMA	IHSMVEY.
PhylomeDB	Q8A684.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2002-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_BACTN

Accession

Primary (citable) accession number: Q8A684

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program