Reviewed, UniProtKB/Swiss-Prot Q8A639 (TYSY_BACTN)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BT_2047

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_0000140933
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A639-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4C66E8C83E3C73F4	FASTA	264	30,392
	10 20 30 40 50 60 MKQYLDLLNR VLTEGTEKSD RTGTGTISVF GHQMRFNLDE GFPCLTTKKL HLKSIIYELL 70 80 90 100 110 120 WFLQGDTNAK YLQEHGVRIW NEWADENGDL GHIYGYQWRS WPDYDGGFID QISEAVETIK 130 140 150 160 170 180 HNPDSRRIIV SAWNVADLKN MNLPPCHAFF QFYVADGRLS LQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLLQMMAQ VTGLKAGEFI HTLGDAHIYL NHLDQVKLQL SREPRALPQM KINPDVKSIY 250 260 DFQFEDFELV NYDPHPHIAG IVAV

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO77154.1.
RefSeq	NP_810960.1.
3D structure databases
SMR	Q8A639. Positions 1-264.
ModBase	Search...
Genome annotation databases
GeneID	1074106.
GenomeReviews	Gene locus BT_2047 in contig AE015928_GR.
KEGG	bth:BT_2047.
NMPDR	fig|226186.1.peg.2047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	Q8A639.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2047-MONOMER.
BRENDA	2.1.1.45. 21018.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACTN

Accession

Primary (citable) accession number: Q8A639

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)