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Q8A624

- PFKA2_BACTN

UniProt

Q8A624 - PFKA2_BACTN

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Protein

ATP-dependent 6-phosphofructokinase 2

Gene

pfkA2

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
Active sitei131 – 1311Proton acceptorUniRule annotation
Binding sitei158 – 1581Allosteric activator ADPUniRule annotation
Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
Binding sitei215 – 2151Allosteric activator ADPUniRule annotation
Binding sitei226 – 2261SubstrateUniRule annotation
Binding sitei250 – 2501Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 762ATPUniRule annotation
Nucleotide bindingi105 – 1084ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-2102-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Alternative name(s):
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:pfkA2UniRule annotation
Ordered Locus Names:BT_2062
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000001414: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326ATP-dependent 6-phosphofructokinase 2PRO_0000111938Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi226186.BT_2062.

Structurei

3D structure databases

ProteinModelPortaliQ8A624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni129 – 1313Substrate bindingUniRule annotation
Regioni173 – 1753Substrate bindingUniRule annotation
Regioni189 – 1913Allosteric activator ADP bindingUniRule annotation
Regioni217 – 2193Allosteric activator ADP bindingUniRule annotation
Regioni256 – 2594Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
InParanoidiQ8A624.
KOiK00850.
OMAiGIYRGYK.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8A624 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGTVKCIGIL TSGGDAPGMN AAIRAVTRAA IYNGLQVKGI YRGYKGLVTG
60 70 80 90 100
EIKEFKSQNV SNIIQLGGTI LKTARCKEFT TPEGRQLAYD NMKREGIDAL
110 120 130 140 150
VIIGGDGSLT GARIFAQEFD VPCIGLPGTI DNDLYGTDTT IGYDTALNTI
160 170 180 190 200
LDAVDKIRDT ATSHERLFFV EVMGRDAGFL ALNGAIASGA EAAIIPEFST
210 220 230 240 250
EVDQLEEFIK NGFRKSKNSS IVLVAESELT GGAMHYAERV KNEYPQYDVR
260 270 280 290 300
VTILGHLQRG GSPTAHDRIL ASRLGAAAID AIMEDQRNVM IGIEHDEIVY
310 320
VPFSKAIKND KPVKRDLVNV LKELSI
Length:326
Mass (Da):35,257
Last modified:June 16, 2003 - v1
Checksum:iEEF46D4915E7A082
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015928 Genomic DNA. Translation: AAO77169.1.
RefSeqiNP_810975.1. NC_004663.1.

Genome annotation databases

EnsemblBacteriaiAAO77169; AAO77169; BT_2062.
GeneIDi1071883.
KEGGibth:BT_2062.
PATRICi21059066. VBIBacThe70966_2120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015928 Genomic DNA. Translation: AAO77169.1 .
RefSeqi NP_810975.1. NC_004663.1.

3D structure databases

ProteinModelPortali Q8A624.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 226186.BT_2062.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO77169 ; AAO77169 ; BT_2062 .
GeneIDi 1071883.
KEGGi bth:BT_2062.
PATRICi 21059066. VBIBacThe70966_2120.

Phylogenomic databases

eggNOGi COG0205.
InParanoidi Q8A624.
KOi K00850.
OMAi GIYRGYK.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci BTHE226186:GJXV-2102-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
    Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
    Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Entry informationi

Entry nameiPFKA2_BACTN
AccessioniPrimary (citable) accession number: Q8A624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3