Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent 6-phosphofructokinase 2

Gene

pfkA2

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase 2 (pfkA2), ATP-dependent 6-phosphofructokinase 3 (pfkA3), ATP-dependent 6-phosphofructokinase 1 (pfkA1)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14ATP; via amide nitrogenUniRule annotation1
Metal bindingi106Magnesium; catalyticUniRule annotation1
Active sitei131Proton acceptorUniRule annotation1
Binding sitei158Allosteric activator ADPUniRule annotation1
Binding sitei166Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei215Allosteric activator ADPUniRule annotation1
Binding sitei226SubstrateUniRule annotation1
Binding sitei250Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 76ATPUniRule annotation2
Nucleotide bindingi105 – 108ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHE:G13PU-7004-MONOMER
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Alternative name(s):
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:pfkA2UniRule annotation
Ordered Locus Names:BT_2062
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119381 – 326ATP-dependent 6-phosphofructokinase 2Add BLAST326

Proteomic databases

PaxDbiQ8A624
PRIDEiQ8A624

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi226186.BT_2062

Structurei

3D structure databases

ProteinModelPortaliQ8A624
SMRiQ8A624
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 28Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni129 – 131Substrate bindingUniRule annotation3
Regioni173 – 175Substrate bindingUniRule annotation3
Regioni189 – 191Allosteric activator ADP bindingUniRule annotation3
Regioni217 – 219Allosteric activator ADP bindingUniRule annotation3
Regioni256 – 259Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
InParanoidiQ8A624
KOiK00850
OMAiGKLHSII
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_00339 Phosphofructokinase_I_B1, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR012828 PFKA_ATP_prok
IPR015912 Phosphofructokinase_CS
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit
TIGRFAMsiTIGR02482 PFKA_ATP, 1 hit
PROSITEiView protein in PROSITE
PS00433 PHOSPHOFRUCTOKINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8A624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTVKCIGIL TSGGDAPGMN AAIRAVTRAA IYNGLQVKGI YRGYKGLVTG
60 70 80 90 100
EIKEFKSQNV SNIIQLGGTI LKTARCKEFT TPEGRQLAYD NMKREGIDAL
110 120 130 140 150
VIIGGDGSLT GARIFAQEFD VPCIGLPGTI DNDLYGTDTT IGYDTALNTI
160 170 180 190 200
LDAVDKIRDT ATSHERLFFV EVMGRDAGFL ALNGAIASGA EAAIIPEFST
210 220 230 240 250
EVDQLEEFIK NGFRKSKNSS IVLVAESELT GGAMHYAERV KNEYPQYDVR
260 270 280 290 300
VTILGHLQRG GSPTAHDRIL ASRLGAAAID AIMEDQRNVM IGIEHDEIVY
310 320
VPFSKAIKND KPVKRDLVNV LKELSI
Length:326
Mass (Da):35,257
Last modified:June 16, 2003 - v1
Checksum:iEEF46D4915E7A082
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA Translation: AAO77169.1
RefSeqiNP_810975.1, NC_004663.1
WP_008761048.1, NC_004663.1

Genome annotation databases

EnsemblBacteriaiAAO77169; AAO77169; BT_2062
GeneIDi1071883
31615509
KEGGibth:BT_2062
PATRICifig|226186.12.peg.2120

Entry informationi

Entry nameiPFKA2_BACTN
AccessioniPrimary (citable) accession number: Q8A624
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health