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Q8A624

- PFKA2_BACTN

UniProt

Q8A624 - PFKA2_BACTN

Protein

ATP-dependent 6-phosphofructokinase 2

Gene

pfkA2

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (16 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
    Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
    Active sitei131 – 1311Proton acceptorUniRule annotation
    Binding sitei158 – 1581Allosteric activator ADPUniRule annotation
    Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei215 – 2151Allosteric activator ADPUniRule annotation
    Binding sitei226 – 2261SubstrateUniRule annotation
    Binding sitei250 – 2501Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 762ATPUniRule annotation
    Nucleotide bindingi105 – 1084ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBTHE226186:GJXV-2102-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 2UniRule annotation
    Short name:
    Phosphofructokinase 2UniRule annotation
    Alternative name(s):
    Phosphohexokinase 2UniRule annotation
    Gene namesi
    Name:pfkA2UniRule annotation
    Ordered Locus Names:BT_2062
    OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
    Taxonomic identifieri226186 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000001414: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326ATP-dependent 6-phosphofructokinase 2PRO_0000111938Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi226186.BT_2062.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8A624.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni129 – 1313Substrate bindingUniRule annotation
    Regioni173 – 1753Substrate bindingUniRule annotation
    Regioni189 – 1913Allosteric activator ADP bindingUniRule annotation
    Regioni217 – 2193Allosteric activator ADP bindingUniRule annotation
    Regioni256 – 2594Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    KOiK00850.
    OMAiGIYRGYK.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8A624-1 [UniParc]FASTAAdd to Basket

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    MGTVKCIGIL TSGGDAPGMN AAIRAVTRAA IYNGLQVKGI YRGYKGLVTG    50
    EIKEFKSQNV SNIIQLGGTI LKTARCKEFT TPEGRQLAYD NMKREGIDAL 100
    VIIGGDGSLT GARIFAQEFD VPCIGLPGTI DNDLYGTDTT IGYDTALNTI 150
    LDAVDKIRDT ATSHERLFFV EVMGRDAGFL ALNGAIASGA EAAIIPEFST 200
    EVDQLEEFIK NGFRKSKNSS IVLVAESELT GGAMHYAERV KNEYPQYDVR 250
    VTILGHLQRG GSPTAHDRIL ASRLGAAAID AIMEDQRNVM IGIEHDEIVY 300
    VPFSKAIKND KPVKRDLVNV LKELSI 326
    Length:326
    Mass (Da):35,257
    Last modified:June 16, 2003 - v1
    Checksum:iEEF46D4915E7A082
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO77169.1.
    RefSeqiNP_810975.1. NC_004663.1.

    Genome annotation databases

    EnsemblBacteriaiAAO77169; AAO77169; BT_2062.
    GeneIDi1071883.
    KEGGibth:BT_2062.
    PATRICi21059066. VBIBacThe70966_2120.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO77169.1 .
    RefSeqi NP_810975.1. NC_004663.1.

    3D structure databases

    ProteinModelPortali Q8A624.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226186.BT_2062.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO77169 ; AAO77169 ; BT_2062 .
    GeneIDi 1071883.
    KEGGi bth:BT_2062.
    PATRICi 21059066. VBIBacThe70966_2120.

    Phylogenomic databases

    eggNOGi COG0205.
    KOi K00850.
    OMAi GIYRGYK.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci BTHE226186:GJXV-2102-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
      Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
      Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

    Entry informationi

    Entry nameiPFKA2_BACTN
    AccessioniPrimary (citable) accession number: Q8A624
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: June 16, 2003
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3