Reviewed, UniProtKB/Swiss-Prot Q8A608 (ILVD_BACTN)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BT_2078

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000103432
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8A608-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 12B379C63791F3E4	FASTA	600	64,045
	10 20 30 40 50 60 MKKQLRSSFS TQGRRMAGAR ALWAANGMKK NQMGKPIIAI VNSFTQFVPG HVHLHEIGQL 70 80 90 100 110 120 VKAEIEKLGC FAAEFNTIAI DDGIAMGHDG MLYSLPSRDI IADSVEYMVN AHKADAMVCI 130 140 150 160 170 180 SNCDKITPGM LMAAMRLNIP TVFVSGGPME AGEWNGQHLD LIDAMIKSAD DSVSDQEVAN 190 200 210 220 230 240 IEQNACPTCG CCSGMFTANS MNCLNEAIGL ALPGNGTIVA THENRTKLFE DAAKLIVENA 250 260 270 280 290 300 MKYYEEGDES VLPRSIATRQ AFLNAMTLDI AMGGSTNTVL HLLAVAHEAG VDFKMDDIDM 310 320 330 340 350 360 LSRKTPCLCK VAPNTQKYHI QDVNRAGGII AILAELAKGG LIDTSVLRVD GMSLAEAIDQ 370 380 390 400 410 420 YSITSPNVTE KAMSKYSSAA GNRFNLVLGS QGAYYQELDK DRANGCIRDL EHAYSKDGGL 430 440 450 460 470 480 AVLKGNIAQD GCVVKTAGVD ESIWKFTGPA KVFDSQEAAC EGILGGRVVS GDVVVITHEG 490 500 510 520 530 540 PKGGPGMQEM LYPTSYIKSR HLGKECALIT DGRFSGGTSG LSIGHVSPEA AAGGNIGKIV 550 560 570 580 590 600 DGDIIEIDIP ARTINVRLTD EELAARPMTP VTRDRYVPKS LKAYASMVSS ADKGAVRLID

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO77185.1.
RefSeq	NP_810991.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1073461.
GenomeReviews	Gene locus BT_2078 in contig AE015928_GR.
KEGG	bth:BT_2078.
NMPDR	fig|226186.1.peg.2078.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A608.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2078-MON.
BRENDA	4.2.1.9. 21018.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACTN

Accession

Primary (citable) accession number: Q8A608

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)