Q8A5V7 (ASNA_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	BT_2129

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195870

Sequences

	Sequence		Length	Mass (Da)
	Q8A5V7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D8190CFB8432742A	FASTA	345	40,120
	10 20 30 40 50 60 MSYLIKPKNY KPLLDLKQTE LGIKQIKEFF QLNLSSELRL RRVTAPLFVL KGMGINDDLN 70 80 90 100 110 120 GIERPVSFPI KDLGDAQAEV VHSLAKWKRL TLADYNIEPG YGIYTDMNAI RSDEELGNLH 130 140 150 160 170 180 SLYVDQWDWE RVITNEDRNV EFLKEIVNRI YAAMIRTEYM VYEMYPQIKP CLPQKLHFIH 190 200 210 220 230 240 SEELRQLYPN LEPKCREHAI CQKYGAVFII GIGCKLSDGK KHDGRAPDYD DYTSTGLNNL 250 260 270 280 290 300 PGLNGDLLLW DDVLQRSIEL SSMGVRVDRE ALQRQLKEEN EEERLKLYFH KRLMDDTLPL 310 320 330 340 SIGGGIGQSR LCMFYLRKAH IGEIQASIWP EDMRKECEEL DIHLI

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO77236.1.
RefSeq	NP_811042.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A5V7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1074292.
GenomeReviews	Gene locus BT_2129 in contig AE015928_GR.
KEGG	bth:BT_2129.
NMPDR	fig|226186.1.peg.2129.
PATRIC	21059210. VBIBacThe70966_2191.
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	Q8A5V7.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2129-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_BACTN

Accession

Primary (citable) accession number: Q8A5V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program