Reviewed, UniProtKB/Swiss-Prot Q8A525 (GUAA2_BACTN)

Names and origin

Protein names

Recommended name:
    Putative GMP synthase [glutamine-hydrolyzing] 2
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase 2
    GMP synthetase 2

Gene names

Name:	guaA2
Ordered Locus Names:	BT_2419

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMP-binding domain.
Caution	Could be the product of a pseudogene. Contains an internal deletion relative to its orthologs.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Putative GMP synthase [glutamine-hydrolyzing] 2 HAMAP MF_00344		PRO_0000140095
Regions
	Domain	1 – 120	120	Glutamine amidotransferase type-1; truncated
	Domain	152 – 311	160	GMP-binding
	Nucleotide binding	148 – 154	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A525-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3CAAB1D45B35D62C	FASTA	435	48,286
	10 20 30 40 50 60 MKQDMIVILD LGSHENTVLA RAIRALGVYS EIYPHDITVE ELKALPNVKG IIINGGLNNV 70 80 90 100 110 120 IDGVAIDVNP SIYTMGIPVM AAGHDKATCA VKLPAFTDDI EAIKAAIKSF VFDTCQAEAN 130 140 150 160 170 180 WNMANFVNDQ IELIRRQVGD KKVLLALSGG VDSSVVAALL LKAIGENLVC VHVNHGLMRK 190 200 210 220 230 240 GESEDVVEVF SNQLKANLVY VDVTDRFLDK LAGVEDPEQK RKIIGGEFIR VFEEEARKLD 250 260 270 280 290 300 GIDFLGQGTI YPDIVESGTK TAKMVKSHHN VGGLPEDLKF QLVEPLRQLF KDEVRACGLE 310 320 330 340 350 360 LGLPYEMVYR QPFPGPGLGV RCLGAITRDR LEAVRESDAI LREEFQIAGL DKKVWQYFTV 370 380 390 400 410 420 VPDFKSVGVR DNARSFDWPV IIRAVNTVDA MTATIEPVDW PILMKITDRI LKEVKNVNRV 430 CYDMSPKPNA TIEWE

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO77526.1.
RefSeq	NP_811332.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ModBase	Search...
Genome annotation databases
GeneID	1073752.
GenomeReviews	Gene locus BT_2419 in contig AE015928_GR.
KEGG	bth:BT_2419.
NMPDR	fig|226186.1.peg.2419.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A525.
OMA	IIKVDAQ.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2419-MON.
BRENDA	6.3.5.2. 21018.
Family and domain databases
HAMAP	MF_00344. Divergent sequence. [Tree]
InterPro	IPR000991. GATase_class1_C. IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. IPR018318. tRNA_MeTrfase-like. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF03054. tRNA_Me_trans. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA2_BACTN

Accession

Primary (citable) accession number: Q8A525

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)