Q8A474 (EFG_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	BT_2729

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Elongation factor G HAMAP MF_00054_B		PRO_0000091072
Regions
	Nucleotide binding	16 – 23	8	GTP By similarity
	Nucleotide binding	84 – 88	5	GTP By similarity
	Nucleotide binding	138 – 141	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A474 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5168F514AF2C7876	FASTA	705	77,587
	10 20 30 40 50 60 MAKTDLHLTR NIGIMAHIDA GKTTTSERIL FYTGLTHKIG EVHDGAATMD WMEQEQERGI 70 80 90 100 110 120 TITSAATTTR WKYAGDTYKI NLIDTPGHVD FTAEVERSLR ILDGAVAAYC AVGGVEPQSE 130 140 150 160 170 180 TVWRQADKYN VPRIAYVNKM DRSGADFFEV VRQMKAVLGA NPCPIVVPIG AEETFKGLVD 190 200 210 220 230 240 LIKMKAIYWH DETMGADYTV EEIPADLVDE ANEWRDKMLE KVAEFDDALM EKYFDDPSTI 250 260 270 280 290 300 TEEEVLRALR NATVQMAVVP MLCGSSFKNK GVQTLLDYVC AFLPSPLDTE NVIGTNPNTG 310 320 330 340 350 360 AEEDRKPSDD EKTSALAFKI ATDPYVGRLT FFRVYSGKIE AGSYIYNSRS GKKERVSRLF 370 380 390 400 410 420 QMHSNKQNPV EVIGAGDIGA GVGFKDIHTG DTLCDETAPI VLESMDFPEP VIGIAVEPKT 430 440 450 460 470 480 QKDMDKLSNG LAKLAEEDPT FTVKTDEQTG QTVISGMGEL HLDIIIDRLK REFKVECNQG 490 500 510 520 530 540 KPQVNYKEAI TKTVDLREVY KKQSGGRGKF ADIIVKIGPV DEDFKEGGLQ FIDEVKGGNI 550 560 570 580 590 600 PKEFIPSVQK GFTSAMKNGV LAGYPLDSMK VTLIDGSFHP VDSDQLSFEI CAIQAYKNAC 610 620 630 640 650 660 AKAGPVLMEP IMKLEVVTPE ENMGDVIGDL NKRRGQVEGM ESSRSGARIV KAMVPLAEMF 670 680 690 700 GYVTALRTIT SGRATSSMVY SHHAQVSNSI AKAVLEEVKG RADLL

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO77835.1.
RefSeq	NP_811641.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A474.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1072301.
GenomeReviews	Gene locus BT_2729 in contig AE015928_GR.
KEGG	bth:BT_2729.
NMPDR	fig|226186.1.peg.2728.
PATRIC	21060401. VBIBacThe70966_2772.
Phylogenomic databases
HOGENOM	HBG737692.
OMA	CNEWREK.
PhylomeDB	Q8A474.
ProtClustDB	PRK12739.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2729-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_BACTN

Accession

Primary (citable) accession number: Q8A474

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program