Q8A455 (SYE_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	BT_2748

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119509
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	260 – 264	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	263	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A455 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B81EB682A53E8157	FASTA	504	57,734
	10 20 30 40 50 60 MAERKVRVRF APSPTGALHI GGVRTALYNY LFARQHGGDL IFRIEDTDSN RFVPGAEEYI 70 80 90 100 110 120 LESFKWLGIH FDEGVSFGGE HGPYRQSERR EIYKKYVQIL LDNDKAYIAF DTPEELDAKR 130 140 150 160 170 180 AEIANFQYDA STRGMMRNSL TMSKEEVDAL IAEGKQYVVR FKIEPNEDVH VNDLIRGEVV 190 200 210 220 230 240 INSSILDDKV LYKSADELPT YHLANIVDDH LMEVSHVIRG EEWLPSAPLH VLLYRAFGWE 250 260 270 280 290 300 DTMPEFAHLP LLLKPEGNGK LSKRDGDRLG FPVFPLEWRP ESGEVSSGYR ESGYLPEAVI 310 320 330 340 350 360 NFLALLGWNP GNDQEVMSMD EMIKLFDIHR CSKSGAKFDY KKGIWFNHQY IQLKPNEEIA 370 380 390 400 410 420 ELFLPVLKEH GVEAPFEKVV TVVGMMKDRV SFIKELWDVC SFFFVAPAEY DEKTVKKRWK 430 440 450 460 470 480 EDSAKCMTEL AEVIAGIEDF SIEGQEKVVM DWIAEKGYHT GNIMNAFRLT LVGEGKGPHM 490 500 FDISWVLGKE ETLARMKRAV EVLK

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO77854.1.
RefSeq	NP_811660.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A455.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1072222.
GenomeReviews	Gene locus BT_2748 in contig AE015928_GR.
KEGG	bth:BT_2748.
NMPDR	fig|226186.1.peg.2747.
PATRIC	21060455. VBIBacThe70966_2795.
Phylogenomic databases
HOGENOM	HBG628189.
OMA	IEWFNLD.
PhylomeDB	Q8A455.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2748-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_BACTN

Accession

Primary (citable) accession number: Q8A455

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program