Reviewed, UniProtKB/Swiss-Prot Q8A407 (SAHH_BACTN)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	BT_2797

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000116943
Regions
	Region	228 – 396	169	NAD binding By similarity
Sites
	Binding site	65	1	Substrate By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	231	1	Substrate By similarity
	Binding site	235	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A407-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 4D11030800F2E163	FASTA	476	52,739
	10 20 30 40 50 60 MIYNMSTELF STLPYKVADI TLADFGRKEI DLAEKEMPGL MALREKYGES KPLKGARIMG 70 80 90 100 110 120 SLHMTIQTAV LIETLVALGA EVRWCSCNIY STQDHAAAAI AASGVAVFAW KGENLADYWW 130 140 150 160 170 180 CTLQALNFPG GKGPNVIVDD GGDATMMIHV GYDAENDAAV LDKEVHAEDE IELNAILKKV 190 200 210 220 230 240 LAEDKTRWHR VAEEMRGVSE ETTTGVHRLY QMQEEGKLLF PAFNVNDSVT KSKFDNLYGC 250 260 270 280 290 300 RESLADGIKR ATDVMIAGKV VVVCGYGDVG KGCSHSMRSY GARVLVTEVD PICALQAAME 310 320 330 340 350 360 GFEVVTMEDA CTEGNIFVTT TGNIDIIRID HMEKMKDQAI VCNIGHFDNE IQVDALKHYS 370 380 390 400 410 420 GIKCVNIKPQ VDRYYFPDGH SILLLADGRL VNLGCATGHP SFVMSNSFTN QTLAQIELFN 430 440 450 460 470 KKYEVNVYRL PKHLDEEVAR LHLEKIGVKL TKLTPEQAAY IGVSVDGPYK AEHYRY

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO77903.1.
RefSeq	NP_811709.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI4 based on UniProtKB P23526.
ModBase	Search...
Genome annotation databases
GeneID	1072485.
GenomeReviews	Gene locus BT_2797 in contig AE015928_GR.
KEGG	bth:BT_2797.
NMPDR	fig|226186.1.peg.2796.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A407.
OMA	Q8A407. HMRAMKD.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2797-MON.
BRENDA	3.3.1.1. 21018.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR000043. Ad_hcy_hydrolase. IPR015878. Ado_hCys_hydrolase_NAD-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.1480. Ad_hcy_hydrolase. 1 hit.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_BACTN

Accession

Primary (citable) accession number: Q8A407

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)