Reviewed, UniProtKB/Swiss-Prot Q8A3M1 (SYM_BACTN)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BT_2933

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	679 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00098
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 679	679	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139103
Regions
	Domain	578 – 679	102	tRNA-binding
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00098
	Motif	332 – 336	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	147	1	Zinc By similarity
	Metal binding	150	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity
	Metal binding	163	1	Zinc By similarity
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A3M1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9F482C9D9B3E922F	FASTA	679	77,647
	10 20 30 40 50 60 MEKKFKRTTV TSALPYANGP VHIGHLAGVY VPADIYVRYL RLKKEDVLFI GGSDEHGVPI 70 80 90 100 110 120 TIRAKKEGVT PQDVVDRYHY LIKKSFEEFG VSFDVYSRTT SKTHHELASD FFKTLYNKGE 130 140 150 160 170 180 FIEKTSEQYY DEEAKTFLAD RYITGECPHC HSEGAYGDQC EKCGTSLSPT DLINPKSAIS 190 200 210 220 230 240 GSKPVMKETK HWYLPLDKHE GWLRQWILED HKEWRPNVYG QCKSWLDMGL QPRAVSRDLD 250 260 270 280 290 300 WGIPVPVEGA EGKVLYVWFD APIGYISNTK ELLPDSWETW WKDPETRLLH FIGKDNIVFH 310 320 330 340 350 360 CIVFPAMLKA EGSYILPDNV PSNEFLNLEG DKISTSRNWA VWLHEYLEDF PGKQDVLRYV 370 380 390 400 410 420 LTANAPETKD NDFTWKDFQA RNNNELVAVY GNFVNRAMVL TQKYFDSKVP ACAELTDYDK 430 440 450 460 470 480 ETLKEFANVK AEVEKLLDVF KFRDAQKEAM NLARIGNKYL ADTEPWKLAK TDMERVGTIL 490 500 510 520 530 540 NISLQLVANL AIAFEPFLPF SSEKLRKMLN MESFEWSELG RNDLLPVGHQ LNKPELLFEK 550 560 570 580 590 600 IEDSVIEAQV QKLLDTKKAN EEANYKANPI RPNIEFDDFT KLDIRVGTIL ECQKVPKADK 610 620 630 640 650 660 LLQFKIDDGL ETRTIVSGIA KHYQPEELVG KQVCFIANLA PRKLKGIVSE GMILSAENND 670 GSLAVIMPQK EVKPGSEVK

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO78039.1.
RefSeq	NP_811845.1.
3D structure databases
HSSP	HSSP built from PDB template 1MKH based on UniProtKB Q9V011.
ModBase	Search...
Genome annotation databases
GeneID	1074000.
GenomeReviews	Gene locus BT_2933 in contig AE015928_GR.
KEGG	bth:BT_2933.
NMPDR	fig|226186.1.peg.2932.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A3M1.
OMA	SAYQPEQ.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_2933-MON.
BRENDA	6.1.1.10. 21018.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BACTN

Accession

Primary (citable) accession number: Q8A3M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)