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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei721 – 7211ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-3190-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BT_3126
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000001414 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 944944Leucine--tRNA ligasePRO_0000151974Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi226186.BT_3126.

Structurei

3D structure databases

ProteinModelPortaliQ8A329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi718 – 7225"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
InParanoidiQ8A329.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8A329-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYNFREIEK KWQQRWVEEK TYQVTEDESK QKFYVLNMFP YPSGAGLHVG
60 70 80 90 100
HPLGYIASDI YARYKRLRGF NVLNPMGYDA YGLPAEQYAI QTGQHPAITT
110 120 130 140 150
KANIDRYREQ LDKIGFSFDW SREIRTCEPE YYHWTQWAFQ KMFNSYYCND
160 170 180 190 200
EQQARPIQEL IDAFAIYGNE GLNAACSEEL SFTAKEWKAK SEKEQQEILM
210 220 230 240 250
NYRIAYLGET MVNWCQALGT VLANDEVIDG VSERGGFPVV QKKMRQWCLR
260 270 280 290 300
VSAYAQRLLD GLDTIDWTES LKETQKNWIG RSEGAEVQFK VKDSDLEFTI
310 320 330 340 350
FTTRADTMFG VTFMVLAPES DLVAQLTTPA QKAEVDAYLD RTKKRTERER
360 370 380 390 400
IADRSVTGVF SGSYAINPFT GEAVPIWISD YVLAGYGTGA IMAVPAHDSR
410 420 430 440 450
DYAFAKHFGL EIRPLVEGCD VSEESFDAKE GIVCNSPRPD VTPYCDLSLN
460 470 480 490 500
GLTIKEAIEK TKQYVKEHNL GRVKVNYRLR DAIFSRQRYW GEPFPVYYKD
510 520 530 540 550
GMPYMIDEDC LPLELPEVDK FLPTETGEPP LGHAKEWAWD TVNKCTVENE
560 570 580 590 600
KIDNVTIFPL ELNTMPGFAG SSAYYLRYMD PHNNKALVDP KVDEYWKNVD
610 620 630 640 650
LYVGGTEHAT GHLIYSRFWN KFLHDVGASV VEEPFQKLVN QGMIQGRSNF
660 670 680 690 700
VYRIKDTHTF VSLNLKDQYE VTPLHVDVNI VSNDILDLEA FKAWRPEYAE
710 720 730 740 750
AEFILEDGKY ICGWAVEKMS KSMFNVVNPD MIVDKYGADT LRMYEMFLGP
760 770 780 790 800
VEQSKPWDTN GIDGVHRFIR KFWSLFYSRT DEYLVKDEPA TKEELKSLHK
810 820 830 840 850
LIKKVTGDIE QFSYNTSVSA FMICVNELSN LKCNKKEILE QLVITLAPFA
860 870 880 890 900
PHVCEELWDT LGHETSVCDA AWPAYNEEYL KEDTINYTIS FNGKARFNME
910 920 930 940
FDADAASDAI QAAVLADERS QKWIEGKTPK KIIVVPKKIV NVVV
Length:944
Mass (Da):108,569
Last modified:June 1, 2003 - v1
Checksum:i551BEBFCD0F19DA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO78232.1.
RefSeqiNP_812038.1. NC_004663.1.
WP_011108648.1. NC_004663.1.

Genome annotation databases

EnsemblBacteriaiAAO78232; AAO78232; BT_3126.
GeneIDi1075992.
KEGGibth:BT_3126.
PATRICi21061253. VBIBacThe70966_3189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO78232.1.
RefSeqiNP_812038.1. NC_004663.1.
WP_011108648.1. NC_004663.1.

3D structure databases

ProteinModelPortaliQ8A329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226186.BT_3126.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO78232; AAO78232; BT_3126.
GeneIDi1075992.
KEGGibth:BT_3126.
PATRICi21061253. VBIBacThe70966_3189.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
InParanoidiQ8A329.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-3190-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
    Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
    Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Entry informationi

Entry nameiSYL_BACTN
AccessioniPrimary (citable) accession number: Q8A329
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.