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Q8A329 (SYL_BACTN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BT_3126
OrganismBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) [Reference proteome] [HAMAP]
Taxonomic identifier226186 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length944 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 944944Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151974

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif718 – 7225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8A329 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 551BEBFCD0F19DA1

FASTA944108,569
        10         20         30         40         50         60 
MEYNFREIEK KWQQRWVEEK TYQVTEDESK QKFYVLNMFP YPSGAGLHVG HPLGYIASDI 

        70         80         90        100        110        120 
YARYKRLRGF NVLNPMGYDA YGLPAEQYAI QTGQHPAITT KANIDRYREQ LDKIGFSFDW 

       130        140        150        160        170        180 
SREIRTCEPE YYHWTQWAFQ KMFNSYYCND EQQARPIQEL IDAFAIYGNE GLNAACSEEL 

       190        200        210        220        230        240 
SFTAKEWKAK SEKEQQEILM NYRIAYLGET MVNWCQALGT VLANDEVIDG VSERGGFPVV 

       250        260        270        280        290        300 
QKKMRQWCLR VSAYAQRLLD GLDTIDWTES LKETQKNWIG RSEGAEVQFK VKDSDLEFTI 

       310        320        330        340        350        360 
FTTRADTMFG VTFMVLAPES DLVAQLTTPA QKAEVDAYLD RTKKRTERER IADRSVTGVF 

       370        380        390        400        410        420 
SGSYAINPFT GEAVPIWISD YVLAGYGTGA IMAVPAHDSR DYAFAKHFGL EIRPLVEGCD 

       430        440        450        460        470        480 
VSEESFDAKE GIVCNSPRPD VTPYCDLSLN GLTIKEAIEK TKQYVKEHNL GRVKVNYRLR 

       490        500        510        520        530        540 
DAIFSRQRYW GEPFPVYYKD GMPYMIDEDC LPLELPEVDK FLPTETGEPP LGHAKEWAWD 

       550        560        570        580        590        600 
TVNKCTVENE KIDNVTIFPL ELNTMPGFAG SSAYYLRYMD PHNNKALVDP KVDEYWKNVD 

       610        620        630        640        650        660 
LYVGGTEHAT GHLIYSRFWN KFLHDVGASV VEEPFQKLVN QGMIQGRSNF VYRIKDTHTF 

       670        680        690        700        710        720 
VSLNLKDQYE VTPLHVDVNI VSNDILDLEA FKAWRPEYAE AEFILEDGKY ICGWAVEKMS 

       730        740        750        760        770        780 
KSMFNVVNPD MIVDKYGADT LRMYEMFLGP VEQSKPWDTN GIDGVHRFIR KFWSLFYSRT 

       790        800        810        820        830        840 
DEYLVKDEPA TKEELKSLHK LIKKVTGDIE QFSYNTSVSA FMICVNELSN LKCNKKEILE 

       850        860        870        880        890        900 
QLVITLAPFA PHVCEELWDT LGHETSVCDA AWPAYNEEYL KEDTINYTIS FNGKARFNME 

       910        920        930        940 
FDADAASDAI QAAVLADERS QKWIEGKTPK KIIVVPKKIV NVVV 

« Hide

References

[1]"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015928 Genomic DNA. Translation: AAO78232.1.
RefSeqNP_812038.1. NC_004663.1.

3D structure databases

ProteinModelPortalQ8A329.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226186.BT_3126.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO78232; AAO78232; BT_3126.
GeneID1075992.
KEGGbth:BT_3126.
PATRIC21061253. VBIBacThe70966_3189.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAERYWMNV.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBTHE226186:GJXV-3190-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BACTN
AccessionPrimary (citable) accession number: Q8A329
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries