Q8A327 (NTPA_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BT_3128

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178130
Regions
	Region	9 – 14	6	Substrate binding By similarity
	Region	70 – 71	2	Substrate binding By similarity
Sites
	Metal binding	70	1	Magnesium or manganese By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A327 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 75D3C83E9C81185B	FASTA	193	21,656
	10 20 30 40 50 60 MKRKLVFATN NAHKLEEIAA ILGDKVELLS LNDIDCHTDI PETAETLEGN ALLKSSFIYR 70 80 90 100 110 120 NYQLDCFADD TGLEVEALNG APGVYSARYA EGEGHDAQAN MRKLLHELEG KENRKAQFRT 130 140 150 160 170 180 AISLILDGKE YLFEGVIKGE IIKEKRGDSG FGYDPIFKPE GYEQTFAELG NETKNKISHR 190 ALAVQKLCEF LQR

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO78234.1.
RefSeq	NP_812040.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A327.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1075977.
GenomeReviews	Gene locus BT_3128 in contig AE015928_GR.
KEGG	bth:BT_3128.
NMPDR	fig|226186.1.peg.3127.
PATRIC	21061257. VBIBacThe70966_3191.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
PhylomeDB	Q8A327.
ProtClustDB	PRK14823.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3128-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BACTN

Accession

Primary (citable) accession number: Q8A327

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program