Reviewed, UniProtKB/Swiss-Prot Q8A2Z5 (GLAA_BACTN)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-galactosidase A
    EC=3.2.1.n1
Alternative name(s):
    Exo-alpha-galactosidase A
    EC=3.2.1.22
    BtGal110A

Gene names

Name:	glaA
Ordered Locus Names:	BT_3160

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues.
Catalytic activity	Hydrolysis of terminal, non-reducing branched alpha-1,3-linked galactose residues. Ref.3 Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. A subfamily. Contains 6 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 568	551	Alpha-1,3-galactosidase A		PRO_0000348473
Regions
	Repeat	87 – 125	39	PbH1 1
	Repeat	243 – 265	23	PbH1 2
	Repeat	299 – 321	23	PbH1 3
	Repeat	409 – 431	23	PbH1 4
	Repeat	432 – 454	23	PbH1 5
	Repeat	465 – 486	22	PbH1 6

Sequences

	Sequence		Length	Mass (Da)
	Q8A2Z5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: BF1CE1BFEAD9EEF3	FASTA	568	65,147
	10 20 30 40 50 60 MMSVWFIQLA IFAQSRIIEV FPEQGKDIEN IALALKKAAD CKGRPVTVKF SPGIYQLDRA 70 80 90 100 110 120 KSSQVLYYIS NTTSELDDPD PTKHIGLYLN TLKNITIDGC GSTLLMNGEM TSFVLDKCEG 130 140 150 160 170 180 IVLKNFNIDY KHPTQTEVEV LEEGNDYLIV QVHPTSQYRI VDAQLEWYGD GWSFKNGIAQ 190 200 210 220 230 240 SYDRISEMTW RSWSPMENLL RTVELRPNVL YLQYKEKPQV GLHTIFQMRD SFRDEVSGFV 250 260 270 280 290 300 NRSKGILLEN INFYYLGNFG VVCQYSENIT VDRCNFAPRP GSGRTNAGFA DFIQVSGCRG 310 320 330 340 350 360 MIDIKNSRFI GAHDDPINIH GTHLRVIEFL SDNRLKLRFM HDQTFGFEAF FKGDDIELVD 370 380 390 400 410 420 SRSLLVVGKC KVKEAKLVTP REMELTLSSP LSSEVMQQKD LVIENVTWTP EVRITNNYFA 430 440 450 460 470 480 RVPTRGILIT TRRKSLIEGN TFYGMQMSGI FVADDGLSWY ESGPVHDLTI RQNTFLNCGE 490 500 510 520 530 540 PIISIDPENR EYKGAVHKNI TIEENYFYMR KNSSCAIRAK AVDGLMIRHN LIYSLDTEKN 550 560 KESDFIQMYN CNEVTIKENR VQLHHLFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacterial glycosidases for the production of universal red blood cells." Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H. Nat. Biotechnol. 25:454-464(2007) [PubMed: 17401360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.
[3]	"Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen." Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H. J. Biol. Chem. 283:8545-8554(2008) [PubMed: 18227066] [Abstract] Cited for: ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM109956 Genomic DNA. Translation: CAJ33352.1. AE015928 Genomic DNA. Translation: AAO78266.1.
RefSeq	NP_812072.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1075907.
GenomeReviews	Gene locus BT_3160 in contig AE015928_GR.
KEGG	bth:BT_3160.
NMPDR	fig|226186.1.peg.3159.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A2Z5.
OMA	LKLRFMH.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3160-MON.
Family and domain databases
InterPro	IPR006626. PbH1. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLAA_BACTN

Accession

Primary (citable) accession number: Q8A2Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)