Q8A2E9 (K6PF3_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase 3
EC=2.7.1.11

Alternative name(s):
Phosphofructokinase 3
Phosphohexokinase 3

Gene names

Name:	pfkA3
Ordered Locus Names:	BT_3356

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) [Reference proteome] [HAMAP]

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides › Bacteroides thetaiotaomicron

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	6-phosphofructokinase 3 HAMAP-Rule MF_00339		PRO_0000111939
Regions
	Nucleotide binding	20 – 24	5	ATP By similarity
	Nucleotide binding	160 – 164	5	ATP By similarity
	Nucleotide binding	177 – 193	17	ATP By similarity
Sites
	Active site	133	1	Proton acceptor By similarity
	Metal binding	191	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
	Binding site	264	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A2E9 [UniParc]. Last modified June 16, 2003. Version 1. Checksum: 990BAEF6B5BD5F79	FASTA	336	35,853
	10 20 30 40 50 60 MRIGILTSGG DCPGINATIR GVCKTAINHY GMEVIGIHSG FQGLLTKDIE SFTDKSLSGL 70 80 90 100 110 120 LNQGGTMLGT SREKPFKKGG VVSDVDKPAL ILQNIQEMGL DCVVCIGGNG TQKTAAKFAA 130 140 150 160 170 180 MGVNIVSVPK TIDNDIWGTD ISFGFDSAVS IATDAIDRLH STASSHKRVM VIEVMGHKAG 190 200 210 220 230 240 WIALYSGMAG GGDVILVPEI PYNIKNIGNT ILERLKKGKP YSIVVVAEGI LTDGRKRAAE 250 260 270 280 290 300 YIAQEIEYET GIETRETVLG YIQRGGSPTP FDRNLSTRMG GHATELIANG QFGRMIALKG 310 320 330 DDISSIPLEE VAGKLKLVTE DHDLVIQGRR MGICFG

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO78462.1.
RefSeq	NP_812268.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A2E9.
ModBase	Search...
Protein-protein interaction databases
STRING	226186.BT_3356.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO78462; AAO78462; BT_3356.
GeneID	1072122.
KEGG	bth:BT_3356.
PATRIC	21061725. VBIBacThe70966_3424.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248869.
KO	K00850.
OMA	NGTQKTA.
ProtClustDB	PRK03202.
Enzyme and pathway databases
BioCyc	BTHE226186:GJXV-3421-MONOMER.
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR022953. Phosphofructokinase. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF3_BACTN

Accession

Primary (citable) accession number: Q8A2E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program