Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8A2E9

- PFKA3_BACTN

UniProt

Q8A2E9 - PFKA3_BACTN

Protein

ATP-dependent 6-phosphofructokinase 3

Gene

pfkA3

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (16 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101ATP; via amide nitrogenUniRule annotation
    Metal bindingi109 – 1091Magnesium; catalyticUniRule annotation
    Sitei110 – 1101Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei133 – 1331Proton acceptorUniRule annotation
    Binding sitei168 – 1681Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei228 – 2281SubstrateUniRule annotation
    Binding sitei255 – 2551Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 732ATPUniRule annotation
    Nucleotide bindingi108 – 1114ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBTHE226186:GJXV-3421-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 3UniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 3UniRule annotation
    Short name:
    Phosphofructokinase 3UniRule annotation
    Alternative name(s):
    Phosphohexokinase 3UniRule annotation
    Gene namesi
    Name:pfkA3UniRule annotation
    Ordered Locus Names:BT_3356
    OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
    Taxonomic identifieri226186 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000001414: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336ATP-dependent 6-phosphofructokinase 3PRO_0000111939Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi226186.BT_3356.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8A2E9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni131 – 1333Substrate bindingUniRule annotation
    Regioni175 – 1773Substrate bindingUniRule annotation
    Regioni261 – 2644Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiVENACIN.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_01976. Phosphofructokinase_III.
    InterProiIPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8A2E9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIGILTSGG DCPGINATIR GVCKTAINHY GMEVIGIHSG FQGLLTKDIE    50
    SFTDKSLSGL LNQGGTMLGT SREKPFKKGG VVSDVDKPAL ILQNIQEMGL 100
    DCVVCIGGNG TQKTAAKFAA MGVNIVSVPK TIDNDIWGTD ISFGFDSAVS 150
    IATDAIDRLH STASSHKRVM VIEVMGHKAG WIALYSGMAG GGDVILVPEI 200
    PYNIKNIGNT ILERLKKGKP YSIVVVAEGI LTDGRKRAAE YIAQEIEYET 250
    GIETRETVLG YIQRGGSPTP FDRNLSTRMG GHATELIANG QFGRMIALKG 300
    DDISSIPLEE VAGKLKLVTE DHDLVIQGRR MGICFG 336
    Length:336
    Mass (Da):35,853
    Last modified:June 16, 2003 - v1
    Checksum:i990BAEF6B5BD5F79
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO78462.1.
    RefSeqiNP_812268.1. NC_004663.1.

    Genome annotation databases

    EnsemblBacteriaiAAO78462; AAO78462; BT_3356.
    GeneIDi1072122.
    KEGGibth:BT_3356.
    PATRICi21061725. VBIBacThe70966_3424.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015928 Genomic DNA. Translation: AAO78462.1 .
    RefSeqi NP_812268.1. NC_004663.1.

    3D structure databases

    ProteinModelPortali Q8A2E9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 226186.BT_3356.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO78462 ; AAO78462 ; BT_3356 .
    GeneIDi 1072122.
    KEGGi bth:BT_3356.
    PATRICi 21061725. VBIBacThe70966_3424.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi VENACIN.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci BTHE226186:GJXV-3421-MONOMER.

    Family and domain databases

    HAMAPi MF_01976. Phosphofructokinase_III.
    InterProi IPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
      Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
      Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

    Entry informationi

    Entry nameiPFKA3_BACTN
    AccessioniPrimary (citable) accession number: Q8A2E9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: June 16, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3