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Protein

ATP-dependent 6-phosphofructokinase 3

Gene

pfkA3

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase 2 (pfkA2), ATP-dependent 6-phosphofructokinase 3 (pfkA3), ATP-dependent 6-phosphofructokinase 1 (pfkA1)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10ATP; via amide nitrogenUniRule annotation1
Metal bindingi109Magnesium; catalyticUniRule annotation1
Sitei110Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei133Proton acceptorUniRule annotation1
Binding sitei168Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei228SubstrateUniRule annotation1
Binding sitei255Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 73ATPUniRule annotation2
Nucleotide bindingi108 – 111ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHE:G13PU-8323-MONOMER
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 3UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 3UniRule annotation
Short name:
Phosphofructokinase 3UniRule annotation
Alternative name(s):
Phosphohexokinase 3UniRule annotation
Gene namesi
Name:pfkA3UniRule annotation
Ordered Locus Names:BT_3356
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119391 – 336ATP-dependent 6-phosphofructokinase 3Add BLAST336

Proteomic databases

PaxDbiQ8A2E9
PRIDEiQ8A2E9

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi226186.BT_3356

Structurei

3D structure databases

ProteinModelPortaliQ8A2E9
SMRiQ8A2E9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 133Substrate bindingUniRule annotation3
Regioni175 – 177Substrate bindingUniRule annotation3
Regioni261 – 264Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ Bacteria
COG0205 LUCA
HOGENOMiHOG000248869
InParanoidiQ8A2E9
KOiK21071
OMAiRDGWRGP
OrthoDBiPOG091H01AC

Family and domain databases

HAMAPiMF_01976 Phosphofructokinase_III, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR000023 Phosphofructokinase_dom
IPR012829 Phosphofructokinase_III
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

Q8A2E9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGILTSGG DCPGINATIR GVCKTAINHY GMEVIGIHSG FQGLLTKDIE
60 70 80 90 100
SFTDKSLSGL LNQGGTMLGT SREKPFKKGG VVSDVDKPAL ILQNIQEMGL
110 120 130 140 150
DCVVCIGGNG TQKTAAKFAA MGVNIVSVPK TIDNDIWGTD ISFGFDSAVS
160 170 180 190 200
IATDAIDRLH STASSHKRVM VIEVMGHKAG WIALYSGMAG GGDVILVPEI
210 220 230 240 250
PYNIKNIGNT ILERLKKGKP YSIVVVAEGI LTDGRKRAAE YIAQEIEYET
260 270 280 290 300
GIETRETVLG YIQRGGSPTP FDRNLSTRMG GHATELIANG QFGRMIALKG
310 320 330
DDISSIPLEE VAGKLKLVTE DHDLVIQGRR MGICFG
Length:336
Mass (Da):35,853
Last modified:June 16, 2003 - v1
Checksum:i990BAEF6B5BD5F79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA Translation: AAO78462.1
RefSeqiNP_812268.1, NC_004663.1
WP_008767561.1, NC_004663.1

Genome annotation databases

EnsemblBacteriaiAAO78462; AAO78462; BT_3356
GeneIDi1072122
31619337
KEGGibth:BT_3356
PATRICifig|226186.12.peg.3424

Similar proteinsi

Entry informationi

Entry nameiPFKA3_BACTN
AccessioniPrimary (citable) accession number: Q8A2E9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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