Reviewed, UniProtKB/Swiss-Prot Q8A2B2 (AROK_BACTN)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	BT_3393

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm Probable HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Shikimate kinase HAMAP MF_00109		PRO_0000237844
Regions
	Nucleotide binding	11 – 16	6	ATP By similarity
Sites
	Metal binding	15	1	Magnesium By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	79	1	Substrate; via amide nitrogen By similarity
	Binding site	118	1	ATP By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A2B2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8309087AFDDBE6B9	FASTA	175	20,504
	10 20 30 40 50 60 MVRIFLTGYM GAGKTTLGKA FARKLNVPFI DLDWYIEERF HKTVGELFTE RGEAGFRELE 70 80 90 100 110 120 RNMLHEVAEF ENVVISTGGG APCFYDNMEF MNRTGKTVFL NVHPDVLFRR LRIAKQQRPI 130 140 150 160 170 LQGKEDDELM DFIIQALEKR APFYTQAQYI FNADELEDRW QIESSVQRLQ ELLEL

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO78499.1.
RefSeq	NP_812305.1.
3D structure databases
SMR	Q8A2B2. Positions 2-174.
ModBase	Search...
Genome annotation databases
GeneID	1074874.
GenomeReviews	Gene locus BT_3393 in contig AE015928_GR.
KEGG	bth:BT_3393.
NMPDR	fig|226186.1.peg.3392.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335815.
OMA	VEKEGWE.
PhylomeDB	Q8A2B2.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3393-MONOMER.
BRENDA	2.7.1.71. 21018.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_BACTN

Accession

Primary (citable) accession number: Q8A2B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	June 1, 2003
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)