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Protein

Shikimate kinase

Gene

aroK

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.UniRule annotation

Catalytic activityi

ATP + shikimate = ADP + shikimate 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (BT_3975)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15MagnesiumUniRule annotation1
Binding sitei33SubstrateUniRule annotation1
Binding sitei57SubstrateUniRule annotation1
Binding sitei79Substrate; via amide nitrogenUniRule annotation1
Binding sitei118ATPUniRule annotation1
Binding sitei140SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate kinaseUniRule annotation (EC:2.7.1.71UniRule annotation)
Short name:
SKUniRule annotation
Gene namesi
Name:aroKUniRule annotation
Ordered Locus Names:BT_3393
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002378441 – 175Shikimate kinaseAdd BLAST175

Proteomic databases

PaxDbiQ8A2B2.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi226186.BT_3393.

Structurei

Secondary structure

1175
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi14 – 25Combined sources12
Beta strandi29 – 31Combined sources3
Helixi32 – 40Combined sources9
Helixi44 – 67Combined sources24
Beta strandi71 – 76Combined sources6
Helixi81 – 83Combined sources3
Helixi87 – 94Combined sources8
Beta strandi95 – 101Combined sources7
Helixi104 – 113Combined sources10
Helixi115 – 117Combined sources3
Helixi119 – 121Combined sources3
Helixi126 – 144Combined sources19
Beta strandi147 – 152Combined sources6
Helixi159 – 172Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VAAX-ray1.70A/B/C1-175[»]
ProteinModelPortaliQ8A2B2.
SMRiQ8A2B2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the shikimate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
HOGENOMiHOG000032569.
InParanoidiQ8A2B2.
KOiK00891.
OMAiTHGEPHF.
OrthoDBiPOG091H02J5.

Family and domain databases

CDDicd00464. SK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8A2B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRIFLTGYM GAGKTTLGKA FARKLNVPFI DLDWYIEERF HKTVGELFTE
60 70 80 90 100
RGEAGFRELE RNMLHEVAEF ENVVISTGGG APCFYDNMEF MNRTGKTVFL
110 120 130 140 150
NVHPDVLFRR LRIAKQQRPI LQGKEDDELM DFIIQALEKR APFYTQAQYI
160 170
FNADELEDRW QIESSVQRLQ ELLEL
Length:175
Mass (Da):20,504
Last modified:June 1, 2003 - v1
Checksum:i8309087AFDDBE6B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO78499.1.
RefSeqiNP_812305.1. NC_004663.1.
WP_008767597.1. NC_004663.1.

Genome annotation databases

EnsemblBacteriaiAAO78499; AAO78499; BT_3393.
GeneIDi1074874.
KEGGibth:BT_3393.
PATRICi21061799. VBIBacThe70966_3461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO78499.1.
RefSeqiNP_812305.1. NC_004663.1.
WP_008767597.1. NC_004663.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VAAX-ray1.70A/B/C1-175[»]
ProteinModelPortaliQ8A2B2.
SMRiQ8A2B2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226186.BT_3393.

Proteomic databases

PaxDbiQ8A2B2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO78499; AAO78499; BT_3393.
GeneIDi1074874.
KEGGibth:BT_3393.
PATRICi21061799. VBIBacThe70966_3461.

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
HOGENOMiHOG000032569.
InParanoidiQ8A2B2.
KOiK00891.
OMAiTHGEPHF.
OrthoDBiPOG091H02J5.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.

Family and domain databases

CDDicd00464. SK. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
[Graphical view]
PfamiPF01202. SKI. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROK_BACTN
AccessioniPrimary (citable) accession number: Q8A2B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.