Reviewed, UniProtKB/Swiss-Prot Q8A294 (HPPA_BACTN)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	BT_3411

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium By similarity. Potassium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 1 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	Pyrophosphate-energized proton pump HAMAP MF_01129		PRO_0000217001
Regions
	Transmembrane	4 – 23	20	Potential
	Transmembrane	58 – 77	20	Potential
	Transmembrane	81 – 103	23	Potential
	Transmembrane	132 – 154	23	Potential
	Transmembrane	169 – 191	23	Potential
	Transmembrane	275 – 297	23	Potential
	Transmembrane	312 – 334	23	Potential
	Transmembrane	339 – 356	18	Potential
	Transmembrane	396 – 418	23	Potential
	Transmembrane	425 – 447	23	Potential
	Transmembrane	486 – 508	23	Potential
	Transmembrane	554 – 576	23	Potential
	Transmembrane	622 – 641	20	Potential
	Transmembrane	643 – 665	23	Potential
	Transmembrane	711 – 733	23	Potential
Sites
	Site	485	1	Determinant of potassium dependence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A294-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 59DA2F60FEC56F46	FASTA	734	76,538
	10 20 30 40 50 60 MDSILFWLVP VASVLALCFA YYFHKQMMKE SEGTPQMIKI AAAVRKGAMS YLKQQYKIVG 70 80 90 100 110 120 CVFLGLVILF SIMAYGFHVQ NVWVPIAFLT GGFFSGLSGF LGMKTATYAS ARTANAARNS 130 140 150 160 170 180 LNSGLRIAFR SGAVMGLVVV GLGLLDISFW YLLLNAVIPA DALTPTHKLC VITTTMLTFG 190 200 210 220 230 240 MGASTQALFA RVGGGIYTKA ADVGADLVGK VEAGIPEDDP RNPATIADNV GDNVGDVAGM 250 260 270 280 290 300 GADLYESYCG SILATAALGA AAFIHSADTV MQFKAVIAPM LIAAVGILLS IIGIFSVRTK 310 320 330 340 350 360 ENATVKDLLG SLAFGTNLSS VLIVAATFLI LWLLQLDNWI WISCAVVVGL LVGIVIGRST 370 380 390 400 410 420 EYYTSQSYRP TQKLSESGKT GPATVIISGI GLGMLSTAIP VIAVVVGIIA SFLLASGFDF 430 440 450 460 470 480 SNVGMGLYGI GIAAVGMLST LGITLATDAY GPIADNAGGN AEMAGLGAEV RKRTDALDSL 490 500 510 520 530 540 GNTTAATGKG FAIGSAALTG LALLASYIEE IRIGLTRLGT VDLSMPNGDV VSTANATFVD 550 560 570 580 590 600 FMNYYDVTLM NPKVLSGMFL GSMMAFLFCG LTMNAVGRAA GHMVDEVRRQ FREIKGILTG 610 620 630 640 650 660 EAEPDYERCV AISTKGAQRE MVIPSLIAIL APIATGLIFG VTGVLGLLIG GLSTGFVLAI 670 680 690 700 710 720 FMANAGGAWD NAKKYVEEGN FGGKGGEVHK ATVVGDTVGD PFKDTSGPSL NILIKLMSMV 730 AIVMAGLTVA WSLF

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO78517.1.
RefSeq	NP_812323.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1072426.
GenomeReviews	Gene locus BT_3411 in contig AE015928_GR.
KEGG	bth:BT_3411.
NMPDR	fig|226186.1.peg.3410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A294.
OMA	KSPGNEK.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3411-MON.
BRENDA	3.6.1.1. 21018.
Family and domain databases
HAMAP	MF_01129. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_BACTN

Accession

Primary (citable) accession number: Q8A294

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)