Reviewed, UniProtKB/Swiss-Prot Q8A256 (MURD_BACTN)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BT_3450

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108970
Regions
	Nucleotide binding	109 – 115	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8A256-1 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: 541FED7F141F0770	FASTA	444	49,492
	10 20 30 40 50 60 MKRIVILGAG ESGAGAAVLA KVKGFETFVS DMSAIKDKYK DLLDSHHIAW EEGHHTEELI 70 80 90 100 110 120 LNADEVIKSP GIPNDAPLIL KLKAQGTPVI SEIEFAGRYT DAKMICITGS NGKTTTTSLI 130 140 150 160 170 180 YHIFKSADLN VGLAGNIGKS LALQVAEEHH DYYIIELSSF QLDNMYNFRA NIAVLMNITP 190 200 210 220 230 240 DHLDRYDHCM QNYIDAKFRI TQNQTTDDAF IFWNDDPIIK QELAKHGLKA HLYPFAAVKE 250 260 270 280 290 300 DGAIAYVEDH EVKITEPIAF NMEQEELALT GQHNLYNSLA AGISANLAGI TKENIRKALS 310 320 330 340 350 360 DFKGVEHRLE KVARVRGIDF INDSKATNVN SCWYALQSMT TKTVLILGGK DKGNDYTEIE 370 380 390 400 410 420 DLVREKCSAL VYLGLHNEKL HAFFDRFGLP VADVQTGMKD AVEAAYKLAK KGETVLLSPC 430 440 CASFDLFKSY EDRGDQFKKY VREL

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO78556.1. Different initiation.
RefSeq	NP_812362.1.
3D structure databases
HSSP	HSSP built from PDB template 1EEH based on UniProtKB P14900.
ModBase	Search...
Genome annotation databases
GeneID	1074391.
GenomeReviews	Gene locus BT_3450 in contig AE015928_GR.
KEGG	bth:BT_3450.
NMPDR	fig|226186.1.peg.3449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A256.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3450-MON.
BRENDA	6.3.2.9. 21018.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BACTN

Accession

Primary (citable) accession number: Q8A256

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	June 16, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)