Reviewed, UniProtKB/Swiss-Prot Q8A255 (MRAY_BACTN)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	BT_3451

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity. HAMAP MF_00038
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00038.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_0000108784
Regions
	Transmembrane	28 – 48	21	Potential
	Transmembrane	71 – 91	21	Potential
	Transmembrane	95 – 115	21	Potential
	Transmembrane	136 – 156	21	Potential
	Transmembrane	211 – 231	21	Potential
	Transmembrane	239 – 259	21	Potential
	Transmembrane	279 – 299	21	Potential
	Transmembrane	313 – 333	21	Potential
	Transmembrane	399 – 419	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8A255-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 83EC40E61372C7BD	FASTA	422	46,833
	10 20 30 40 50 60 MLYYLFEWLH KLNFPGAGMF GYTSFRALMA VILALLISSI WGDKFINLLK KKQITETQRD 70 80 90 100 110 120 AKTDPFGVNK VGVPSMGGVI IIVAILIPCL LLGKLDNIYM ILMLITTVWL GSLGFADDYI 130 140 150 160 170 180 KIFKKDKEGL HGKFKIIGQV GLGLIVGLTL YLSPDVVIRE NIEVHTPGQE MEVIHGTNDL 190 200 210 220 230 240 KSTQTTIPFF KSNNLDYADL VGFMGEHAQT AGWFLFVIIT IFVVTAVSNG ANLNDGMDGM 250 260 270 280 290 300 AAGNSAIIGA TLGILAYVSS HIEFASYLNI MYIPGSEELV IYICAFIGAL IGFLWYNAYP 310 320 330 340 350 360 AQVFMGDTGS LTIGGIIAVF AIIIHKELLI PILCGVFLVE NLSVILQRFY YKIGKRKGVK 370 380 390 400 410 420 QRLFKRTPIH DHFRTSMSLV EPGCTVKFTK PDQLFHESKI TVRFWIVTIV LAAITIITLK IR

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO78557.1.
RefSeq	NP_812363.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1074265.
GenomeReviews	Gene locus BT_3451 in contig AE015928_GR.
KEGG	bth:BT_3451.
NMPDR	fig|226186.1.peg.3450.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG708263.
OMA	MLITTIW.
PhylomeDB	Q8A255.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3451-MONOMER.
BRENDA	2.7.8.13. 21018.
Family and domain databases
HAMAP	MF_00038. MraY. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BACTN

Accession

Primary (citable) accession number: Q8A255

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)