Reviewed, UniProtKB/Swiss-Prot Q8A254 (MURE_BACTN)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	BT_3452

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_0000101867
Regions
	Nucleotide binding	111 – 117	7	ATP Potential
	Region	153 – 154	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	402 – 405	4	Meso-diaminopimelate binding By similarity
	Motif	402 – 405	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	30	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	180	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	186	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	188	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	378	1	Meso-diaminopimelate By similarity
	Binding site	455	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	459	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	220	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A254-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B9118A18DF56614B	FASTA	482	52,932
	10 20 30 40 50 60 MLLNELLKAI QPVQVTGDSR IEITGVNIDS RLVEAGQLFM AMRGTQTDGH AYIPAAIQKG 70 80 90 100 110 120 ATAILCEDIP EEPVAGITYI QVKDSEDAVG KIATTFYGNP TSQFELVGVT GTNGKTTIAT 130 140 150 160 170 180 LLYNTFRYFG YKVGLISTVC NYIDDQPIPT EHTTPDPITL NRLLGQMADE GCKYVFMEVS 190 200 210 220 230 240 SHSIAQKRIS GLKFAGGIFT NLTRDHLDYH KTVENYLKAK KKFFDDMPKN AFSLTNLDDK 250 260 270 280 290 300 NGLVMTQNTR SKVYTYSLRS LSDFKGRVIE SHFEGMLLDF NNHELAVQFI GKFNASNLLA 310 320 330 340 350 360 VFGAAVLLGK KEEDVLLALS TLHPVAGRFD AIRSPKGVTA IVDYAHTPDA LVNVLNAIHG 370 380 390 400 410 420 VLEGKGKVIT VVGAGGNRDK GKRPIMAKEA AKASDRVIIT SDNPRFEEPQ EIINDMLAGL 430 440 450 460 470 480 DAEDMKKTLS IADRKEAIRT ACMLAEKGDV ILVAGKGHEN YQEIKGVKHH FDDKEVLKEI FN

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO78558.1.
RefSeq	NP_812364.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1073969.
GenomeReviews	Gene locus BT_3452 in contig AE015928_GR.
KEGG	bth:BT_3452.
NMPDR	fig|226186.1.peg.3451.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A254.
OMA	FPVIVDY.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3452-MON.
BRENDA	6.3.2.13. 21018.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_BACTN

Accession

Primary (citable) accession number: Q8A254

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)