Reviewed, UniProtKB/Swiss-Prot Q8A245 (DUT_BACTN)

Names and origin

Protein names

Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase
      Short name=dUTPase
    EC=3.6.1.23
Alternative name(s):
    dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	BT_3461

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 144	144	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_0000182826
Regions
	Region	63 – 65	3	Substrate binding By similarity
	Region	80 – 82	3	Substrate binding By similarity
Sites
	Binding site	76	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A245-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 77B093540F371178	FASTA	144	15,590
	10 20 30 40 50 60 MNVQVINKSK HPLPAYATEL SAGMDIRANL SEPITLAPLQ RCLVPTGIYI ALPQGFEAQV 70 80 90 100 110 120 RPRSGLAIKK GITVLNSPGT IDADYRGEVC IILVNLSSEP FVIEDGERIA QMVIARHEQA 130 140 VWQEVEVLDE TERGAGGFGH TGRG

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO78567.1.
RefSeq	NP_812373.1.
3D structure databases
SMR	Q8A245. Positions 3-127.
ModBase	Search...
Genome annotation databases
GeneID	1075999.
GenomeReviews	Gene locus BT_3461 in contig AE015928_GR.
KEGG	bth:BT_3461.
NMPDR	fig|226186.1.peg.3460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG436079.
OMA	IAQMVFA.
PhylomeDB	Q8A245.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3461-MONOMER.
BRENDA	3.6.1.23. 21018.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. DeoxyUTP_pyroPase_dom. IPR008181. dUTP_pyrophosphatase_subfam_1. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BACTN

Accession

Primary (citable) accession number: Q8A245

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)