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Q8A1F3 (DDL_BACTN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:BT_3713
OrganismBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) [Reference proteome] [HAMAP]
Taxonomic identifier226186 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177786

Regions

Domain121 – 321201ATP-grasp
Nucleotide binding149 – 20456ATP By similarity

Sites

Metal binding2751Magnesium or manganese 1 By similarity
Metal binding2881Magnesium or manganese 1 By similarity
Metal binding2881Magnesium or manganese 2 By similarity
Metal binding2901Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8A1F3 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: FFA3A4D7FC8D170A

FASTA32436,106
        10         20         30         40         50         60 
MKRTIAIVAG GDTSEFHVSL RSAQGIYSFI DKEKYTLYIV EMQGTRWEVQ LPDGAKAPVD 

        70         80         90        100        110        120 
RNDFSFMLGT EKIRFDFAYI TIHGTPGEDG RLQGYFDMMH IPYSCCGVLA AAITYDKFTC 

       130        140        150        160        170        180 
NQYLKAFGVR IAESLLLRQG QSVSDEDVME KIGLPCFIKP SLGGSSFGVT KVKTKEQIQP 

       190        200        210        220        230        240 
AIVKAFEEAQ EVLVEAFMEG TELTCGCYKT KDKTVIFPPT EVVTHNEFFD YDAKYNGQVD 

       250        260        270        280        290        300 
EITPARISDE LTNRVQMLTS AIYDILGCSG IIRVDYIVTA GEKINLLEVN TTPGMTTTSF 

       310        320 
IPQQVRAAGL DIKDVMTDII ENKF 

« Hide

References

[1]"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015928 Genomic DNA. Translation: AAO78818.1.
RefSeqNP_812624.1. NC_004663.1.

3D structure databases

ProteinModelPortalQ8A1F3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING226186.BT_3713.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO78818; AAO78818; BT_3713.
GeneID1072146.
KEGGbth:BT_3713.
PATRIC21062427. VBIBacThe70966_3773.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAMEFCVLF.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycBTHE226186:GJXV-3779-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BACTN
AccessionPrimary (citable) accession number: Q8A1F3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways