Reviewed, UniProtKB/Swiss-Prot Q8A1E8 (PROA_BACTN)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BT_3718

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189697

Sequences

	Sequence		Length	Mass (Da)
	Q8A1E8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2B2D91E6B4D7A8C5	FASTA	417	45,645
	10 20 30 40 50 60 MTTNLNGTFA AVQAASRELA LLSDDTINQI LNAVADATIA ETSFILSENE KDLARMDKSN 70 80 90 100 110 120 PKYDRLKLTE ERLKGIAADT RNVATLPSPL GRILKETSRP NGMKLTKVSV PFGVIGIIYE 130 140 150 160 170 180 ARPNVSFDVF SLCLKSGNAC ILKGGSDADD SNRAIISVIH KVLEKFHVNP HIVELLPADR 190 200 210 220 230 240 EATAALLNAT GYVDLIIPRG SSNLINFVRE NARIPVIETG AGICHTYFDE FGDTRKGADI 250 260 270 280 290 300 IHNAKTRRVS VCNALDCTII HEKRLGDLPA LCDQLKESKV TIYADTQAYQ ALEGYYPAEL 310 320 330 340 350 360 LQPATPESFG TEFLDYKMAV KTVKSFEDAL GHIQENSSRH SECIVTENKE RATLFTKIVD 370 380 390 400 410 AACVYTNVST AFTDGAQFGL GAEIGISTQK LHARGPMGLE EITSYKWVIE GDGQTRW

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO78823.1.
RefSeq	NP_812629.1.
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Genome annotation databases
GeneID	1075939.
GenomeReviews	Gene locus BT_3718 in contig AE015928_GR.
KEGG	bth:BT_3718.
NMPDR	fig|226186.1.peg.3716.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A1E8.
OMA	Q8A1E8. QYPAACN.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3718-MON.
BRENDA	1.2.1.41. 21018.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BACTN

Accession

Primary (citable) accession number: Q8A1E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)