Reviewed, UniProtKB/Swiss-Prot Q8A1E7 (PROB_BACTN)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BT_3719

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109643
Regions
	Domain	278 – 356	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q8A1E7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7DFF9BB6EB5CBC02	FASTA	360	39,454
	10 20 30 40 50 60 MKQEFTRIAV KVGSNVLARR DGTLDVTRMS ALVDQIAELN KSGVEIILIS SGAVASGRSE 70 80 90 100 110 120 IHPQKKLDSV DQRQLFSAVG QAKLINRYYE LFREHGIAVG QVLTTKENFS TRRHYLNQKN 130 140 150 160 170 180 CMTVMLENGV IPIVNENDTI SVSELMFTDN DELSGLIASM MDAQALIILS NIDGIYNGSP 190 200 210 220 230 240 SDPASAVIRE IEHGKDLSNY IQATKSSFGR GGMLTKTNIA RKVADEGITV IIANGKRDNI 250 260 270 280 290 300 LVDLLQQPDD TVCTRFIPST EAVSSVKKWI AHSEGFAKGE IHINECATDI LSSEKAASIL 310 320 330 340 350 360 PVGITHIEGE FEKDDIVRIM DFLGNQVGVG KANCDSAQAR EAMGKHGKKP VVHYDYLYIE

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO78824.1.
RefSeq	NP_812630.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1072190.
GenomeReviews	Gene locus BT_3719 in contig AE015928_GR.
KEGG	bth:BT_3719.
NMPDR	fig|226186.1.peg.3717.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A1E7.
OMA	ELMHIYS.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3719-MON.
BRENDA	2.7.2.11. 21018.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BACTN

Accession

Primary (citable) accession number: Q8A1E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)