Q8A1D5 (PYRE_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BT_3731

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110673
Regions
	Region	123 – 131	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	97	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	101	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	103	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	127	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A1D5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7A6B89BBB7346872	FASTA	212	23,453
	10 20 30 40 50 60 MKNLERLFAE KLLKIKAIKL QPANPFTWAS GWKSPFYCDN RKTLSYPSLR NFVKIEITRL 70 80 90 100 110 120 ILERFGQVDA IAGVATGAIP QGALVADALN LPFVYVRSTP KDHGLENLIE GELRPGMKVV 130 140 150 160 170 180 VVEDLISTGG SSLKAVEAIR RDGCEVIGMV AAYTYGFPVA EEAFKNAKVP LVTLTNYEAV 190 200 210 LDVALRTGYI EEEDIATLND WRKDPAHWDA GK

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO78836.1.
RefSeq	NP_812642.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A1D5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1072336.
GenomeReviews	Gene locus BT_3731 in contig AE015928_GR.
KEGG	bth:BT_3731.
NMPDR	fig|226186.1.peg.3729.
PATRIC	21062465. VBIBacThe70966_3792.
Phylogenomic databases
HOGENOM	HBG404341.
OMA	LPMTYVR.
PhylomeDB	Q8A1D5.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3731-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BACTN

Accession

Primary (citable) accession number: Q8A1D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program