Reviewed, UniProtKB/Swiss-Prot Q8A155 (PUR9_BACTN)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	BT_3812

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity.
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_0000192071

Sequences

	Sequence		Length	Mass (Da)
	Q8A155-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 0C09966527E570E4	FASTA	507	55,880
	10 20 30 40 50 60 MSESKRIKTA LVSVYHKEGL DEIITKLYEE GVEFLSTGGT RQFIESLGYP CKAVEDLTTY 70 80 90 100 110 120 PSILGGRVKT LHPKIFGGIL CRRDLEQDIQ QIEKYEIPEI DLVIVDLYPF EATVASGASE 130 140 150 160 170 180 ADIIEKIDIG GISLIRAAAK NYNDVIIVAS QAQYKPLLDM LMEHGATSSL EERRWMAKEA 190 200 210 220 230 240 FAVSSHYDSA IFNYFDAGEG SAFRCSVNNQ KQLRYGENPH QKGYFYGNLD AMFDQIHGKE 250 260 270 280 290 300 ISYNNLLDIN AAVDLIDEYE DLTFAILKHN NACGLASRPT VLEAWTDALA GDPVSAFGGV 310 320 330 340 350 360 LITNGVIDKA AAEEINKIFF EVIIAPDYDV DALEILGQKK NRIILVRKEA KLPKKQFRAL 370 380 390 400 410 420 LNGVLVQDKD MNIETVADLR TVTDKAPTPE EVEDLLFANK IVKNSKSNAI VLAKGKQLLA 430 440 450 460 470 480 SGVGQTSRVD ALKQAIEKAK SFGFDLNGAV MASDAFFPFP DCVEIADKEG ITAVIQPGGS 490 500 VKDDLTFAYC NEHGMAMVTT GIRHFKH

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO78917.1.
RefSeq	NP_812723.1.
3D structure databases
HSSP	HSSP built from PDB template 1G8M based on UniProtKB P31335.
ModBase	Search...
Genome annotation databases
GeneID	1072684.
GenomeReviews	Gene locus BT_3812 in contig AE015928_GR.
KEGG	bth:BT_3812.
NMPDR	fig|226186.1.peg.3810.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A155.
OMA	Q8A155. VILAHEY.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3812-MON.
BRENDA	2.1.2.3. 21018. 3.5.4.10. 21018.
Family and domain databases
HAMAP	MF_00139. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHas_formly. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR9_BACTN

Accession

Primary (citable) accession number: Q8A155

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)