Q8A0Z8 (SYN_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	BT_3873

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Asparagine--tRNA ligase HAMAP MF_00534		PRO_0000176394

Sequences

	Sequence		Length	Mass (Da)
	Q8A0Z8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4D3F1E9594A5F2EC	FASTA	467	53,295
	10 20 30 40 50 60 MEKIGRTKIV DLLKREDIGA MVNVKGWVRT RRGSKQVNFI ALNDGSTINN VQIVVDLANF 70 80 90 100 110 120 DEEMLKLITT GACISVNGVM VESVGSGQKV EVQAKEIEVL GTCDNTYPLQ KKGHSMEFLR 130 140 150 160 170 180 EIAHLRPRTN TFGAVFRIRH NMAIAIHKFF HEKGFFYFHT PIITGSDCEG AGQMFQVTTM 190 200 210 220 230 240 NLYDLKKDER GSISYDDDFF GKQASLTVSG QLEGELAATA LGAIYTFGPT FRAENSNTPR 250 260 270 280 290 300 HLAEFWMIEP EVAFNDIADN MDLAEEFIKY CVKWALDNCA DDVKFLNDMF DKGLIERLQG 310 320 330 340 350 360 VLKDDFVRLP YTDGIKILED AVAKGHKFEF PVYWGVDLAS EHERYLVEEH FKRPVILTDY 370 380 390 400 410 420 PKEIKAFYMK QNEDGKTVRA MDVLFPKIGE IIGGSEREAD YNKLMTRIEE MHIPMKDMWW 430 440 450 460 YLDTRKFGTC PHSGFGLGFE RLLLFVTGMS NIRDVIPFPR TPRNADF

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO78978.1.
RefSeq	NP_812784.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A0Z8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1072981.
GenomeReviews	Gene locus BT_3873 in contig AE015928_GR.
KEGG	bth:BT_3873.
NMPDR	fig|226186.1.peg.3871.
PATRIC	21062776. VBIBacThe70966_3937.
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	Q8A0Z8.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3873-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BACTN

Accession

Primary (citable) accession number: Q8A0Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program