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Q8A0X7 (TRMB_BACTN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BT_3894
OrganismBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) [Reference proteome] [HAMAP]
Taxonomic identifier226186 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides ›

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Sequence caution

The sequence AAO78999.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 252252tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171296

Regions

Region199 – 2024Substrate binding Potential

Sites

Binding site511S-adenosyl-L-methionine By similarity
Binding site761S-adenosyl-L-methionine By similarity
Binding site1031S-adenosyl-L-methionine By similarity
Binding site1251S-adenosyl-L-methionine By similarity
Binding site1591Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q8A0X7 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: EA78C50BFAA35710

FASTA25229,494
        10         20         30         40         50         60 
MGKNKLEKFA DMASYPHVFE YPYSAVDNVP FDMKGKWHQE FFGNDHPIVL ELGCGRGEYT 

        70         80         90        100        110        120 
VGLGRMFPDK NFIAVDIKGS RMWTGATESL QAGMKNVAFL RTNIEIIERF FAAGEVSEIW 

       130        140        150        160        170        180 
LTFSDPQMKK ATKRLTSTYF MERYRKFLKP DGIIHLKTDS NFMFTYTKYM IEANQLPVEF 

       190        200        210        220        230        240 
ITEDLYHSDL VDDILSIKTY YEQQWLDRGL NIKYIKFRLP QEGVLQEPDV EIELDPYRSY 

       250 
NRSKRSGLQT SK

« Hide

References

[1]"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE015928 Genomic DNA. Translation: AAO78999.1. Different initiation.
RefSeqNP_812805.1. NC_004663.1.

3D structure databases

ProteinModelPortalQ8A0X7.
ModBaseSearch...

Protein-protein interaction databases

STRING226186.BT_3894.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO78999; AAO78999; BT_3894.
GeneID1073797.
KEGGbth:BT_3894.
PATRIC21062820. VBIBacThe70966_3959.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAVFQPTRE.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycBTHE226186:GJXV-3970-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BACTN
AccessionPrimary (citable) accession number: Q8A0X7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: May 29, 2007
Last modified: May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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