Q8A0U8 (ISPD_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	BT_3923

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000075552
Sites
	Site	15	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	151	1	Positions MEP for the nucleophilic attack By similarity
	Site	205	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A0U8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7291A5226555E55A	FASTA	219	24,381
	10 20 30 40 50 60 MKQSVIIVAG GKGLRMGSDL PKQFLPVGGK PVLMHTLEAF RKYDAMLQII LVLPREQQDF 70 80 90 100 110 120 WKQLCEEHHF SVEHLVADGG ETRFHSVKNG LALVQAPGLV GVHDGVRPFV TLEVIRRCYE 130 140 150 160 170 180 LAEQHKAVIP VVDVVETLRH LTDAGSETVS RTEYKLVQTP QVFEVELLKQ AYGQEFTPFF 190 200 210 TDDASVVEAM GVPVHLAEGN RENIKITTPF DLKIGSALL

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO79028.1.
RefSeq	NP_812834.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A0U8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1073045.
GenomeReviews	Gene locus BT_3923 in contig AE015928_GR.
KEGG	bth:BT_3923.
NMPDR	fig|226186.1.peg.3921.
PATRIC	21062876. VBIBacThe70966_3987.
Phylogenomic databases
HOGENOM	HBG672839.
OMA	REQQDFW.
PhylomeDB	Q8A0U8.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_3923-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_BACTN

Accession

Primary (citable) accession number: Q8A0U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 4, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program