Q8A0F4 (NUOA_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit A
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit A
NDH-1 subunit A
NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	BT_4067

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 116	116	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_0000362628
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Transmembrane	88 – 108	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8A0F4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: FEAB1CA132E1DF48	FASTA	116	13,286
	10 20 30 40 50 60 MNFTFLVVVL LTALAFVGVV IALSRAISPR SYNVQKFEAY ECGIPTRGKS WMQFRVGYYL 70 80 90 100 110 FAILFLMFDV ETAFLFPWAV VMHDMGPQGL VSILFFFIIL VLGLAYAWRK GALEWK

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO79172.1.
RefSeq	NP_812978.1. NC_004663.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1073431.
GenomeReviews	Gene locus BT_4067 in contig AE015928_GR.
KEGG	bth:BT_4067.
NMPDR	fig|226186.1.peg.4065.
PATRIC	21063170. VBIBacThe70966_4132.
Phylogenomic databases
HOGENOM	HBG605540.
OMA	GERELTY.
PhylomeDB	Q8A0F4.
ProtClustDB	CLSK823774.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_4067-MONOMER.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR023043. NAD(P)H_OxRDtase_bac/plastid. IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
KO	K00330.
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_BACTN

Accession

Primary (citable) accession number: Q8A0F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program