Reviewed, UniProtKB/Swiss-Prot Q8A0C2 (DXS_BACTN)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BT_4099

Organism

Bacteroides thetaiotaomicron [Complete proteome] [HAMAP]

Taxonomic identifier

818 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189087

Sequences

	Sequence		Length	Mass (Da)
	Q8A0C2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2AB8C3C44436A71E	FASTA	647	71,645
	10 20 30 40 50 60 MKNEPIYNLL NSINSPDDLR RLEVDQLPEV CDELRQDIIK ELSCNPGHFA ASLGTVELTV 70 80 90 100 110 120 ALHYVYNTPY DRIVWDVGHQ AYGHKILTGR REAFSTNRKL GGIRPFPSPE ESEYDTFTCG 130 140 150 160 170 180 HASNSISAAL GMAVAAAKKG DDQRHVIAII GDGSMSGGLA FEGLNNSSTT PNNLLIILND 190 200 210 220 230 240 NDMAIDRSVG GMKQYLFNLT TSNRYNQLRF KASRLLFKLG ILNDERRKAL IRFGNSLKSM 250 260 270 280 290 300 AAQQQNIFEG MNIRYFGPID GHDIKNLSRV LRDIKDLKGP KILHLHTIKG KGFAPAEKHA 310 320 330 340 350 360 TEWHAPGKFD PVTGERFVAN TEGMPPLFQD VFGNTLVELA EANPRIVGVT PAMPSGCSMN 370 380 390 400 410 420 ILMSKMPKRA FDVGIAEGHA VTFSGGMAKD GLQPFCNIYS SFMQRAYDNI IHDVAIQNLP 430 440 450 460 470 480 VVLCLDRAGL VGEDGPTHHG AFDMAYLRPI PNLTIASPMN EHELRRLMYT AQLPDKGPFV 490 500 510 520 530 540 LRYPRGRGVL VDWKCPLEEI PVGKGRKLKD GKDIAVISIG PIGNKARSAI ARAESESGRS 550 560 570 580 590 600 IAHYDLRFLK PLDEELLHEV GRTFRHIVTI EDGTIQGGMG SAVLEFMADH EYTPTVKRIG 610 620 630 640 IPDKFVQHGT VAELYQLCGM DEDSLTKELL KQCELLPDMS KIKELTN

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015928 Genomic DNA. Translation: AAO79204.1.
RefSeq	NP_813010.1.
3D structure databases
HSSP	HSSP built from PDB template 1IK6 based on UniProtKB Q8ZUR7.
ModBase	Search...
Genome annotation databases
GeneID	1074692.
GenomeReviews	Gene locus BT_4099 in contig AE015928_GR.
KEGG	bth:BT_4099.
NMPDR	fig|226186.1.peg.4097.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8A0C2.
OMA	MQELPHP.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_4099-MON.
BRENDA	2.2.1.7. 21018.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. False negative. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACTN

Accession

Primary (citable) accession number: Q8A0C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)