Q8A029 (LIPA_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase
EC=2.8.1.8

Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA

Gene names

Name:	lipA
Ordered Locus Names:	BT_4192

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) [Reference proteome] [HAMAP]

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides › Bacteroides thetaiotaomicron

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00206.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Lipoyl synthase HAMAP-Rule MF_00206		PRO_0000102289
Sites
	Metal binding	37	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	42	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	48	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	63	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	67	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	70	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8A029 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F3E992E4F24F728C	FASTA	282	31,352
	10 20 30 40 50 60 MADRVRKPEW LKINIGANDR YTETKRIVDS HCLHTICSSG RCPNMGECWG KGTATFMIGG 70 80 90 100 110 120 DICTRSCKFC NTQTGRPHPL DANEPTHVAE SIALMKLDHA VVTSVDRDDL PDLGAGHWAH 130 140 150 160 170 180 TIREIKRLNP QTTIEVLIPD FQGRMELVDL VIEANPDIIS HNMETVRRIS PLVRSAANYD 190 200 210 220 230 240 TSLQVIGHIA RSGTKSKSGI MVGLGETPQE VETIMDDLLA VGCQILTIGQ YLQPTHRHYP 250 260 270 280 VAEYVTPQQF ATYKTIGLEK GFSIVESAPL VRSSYHAEKH IR

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO79297.1.
RefSeq	NP_813103.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q8A029.
ModBase	Search...
Protein-protein interaction databases
STRING	226186.BT_4192.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO79297; AAO79297; BT_4192.
GeneID	1072478.
KEGG	bth:BT_4192.
PATRIC	21063426. VBIBacThe70966_4260.
Phylogenomic databases
eggNOG	COG0320.
HOGENOM	HOG000235998.
KO	K03644.
OMA	EEYVTPE.
ProtClustDB	PRK05481.
Enzyme and pathway databases
BioCyc	BTHE226186:GJXV-4270-MONOMER.
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIPA_BACTN

Accession

Primary (citable) accession number: Q8A029

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program