ID   Q89ZY0_BACTN            Unreviewed;      1103 AA.
AC   Q89ZY0;
DT   01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2003, sequence version 1.
DT   27-MAR-2024, entry version 133.
DE   RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE            EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
DE   AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230};
GN   OrderedLocusNames=BT_4241 {ECO:0000313|EMBL:AAO79346.1};
OS   Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 /
OS   CCUG 10774 / NCTC 10582 / VPI-5482 / E50).
OC   Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Bacteroides.
OX   NCBI_TaxID=226186 {ECO:0000313|EMBL:AAO79346.1, ECO:0000313|Proteomes:UP000001414};
RN   [1] {ECO:0000313|EMBL:AAO79346.1, ECO:0000313|Proteomes:UP000001414}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC   VPI-5482 / E50 {ECO:0000313|Proteomes:UP000001414};
RX   PubMed=12663928; DOI=10.1126/science.1080029;
RA   Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C.,
RA   Hooper L.V., Gordon J.I.;
RT   "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis.";
RL   Science 299:2074-2076(2003).
RN   [2] {ECO:0000313|EMBL:AAO79346.1, ECO:0000313|Proteomes:UP000001414}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC   VPI-5482 / E50 {ECO:0000313|Proteomes:UP000001414};
RX   PubMed=19321416; DOI=10.1073/pnas.0901529106;
RA   Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S.,
RA   Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L.,
RA   Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C.,
RA   Hettich R.L., Gordon J.I.;
RT   "Characterizing a model human gut microbiota composed of members of its two
RT   dominant bacterial phyla.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|ARBA:ARBA00001412};
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC       {ECO:0000256|ARBA:ARBA00007401}.
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DR   EMBL; AE015928; AAO79346.1; -; Genomic_DNA.
DR   RefSeq; NP_813152.1; NC_004663.1.
DR   AlphaFoldDB; Q89ZY0; -.
DR   SMR; Q89ZY0; -.
DR   STRING; 226186.BT_4241; -.
DR   CAZy; GH2; Glycoside Hydrolase Family 2.
DR   PaxDb; 226186-BT_4241; -.
DR   EnsemblBacteria; AAO79346; AAO79346; BT_4241.
DR   KEGG; bth:BT_4241; -.
DR   PATRIC; fig|226186.12.peg.4312; -.
DR   eggNOG; COG3250; Bacteria.
DR   HOGENOM; CLU_002346_1_1_10; -.
DR   InParanoid; Q89ZY0; -.
DR   OrthoDB; 9801077at2; -.
DR   Proteomes; UP000001414; Chromosome.
DR   GO; GO:0009341; C:beta-galactosidase complex; IBA:GO_Central.
DR   GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0005990; P:lactose catabolic process; IBA:GO_Central.
DR   Gene3D; 2.70.98.10; -; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR004199; B-gal_small/dom_5.
DR   InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR006101; Glyco_hydro_2.
DR   InterPro; IPR006103; Glyco_hydro_2_cat.
DR   InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR   InterPro; IPR006104; Glyco_hydro_2_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR032312; LacZ_4.
DR   PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR   Pfam; PF02929; Bgal_small_N; 1.
DR   Pfam; PF00703; Glyco_hydro_2; 1.
DR   Pfam; PF02836; Glyco_hydro_2_C; 1.
DR   Pfam; PF02837; Glyco_hydro_2_N; 1.
DR   Pfam; PF16353; LacZ_4; 1.
DR   PRINTS; PR00132; GLHYDRLASE2.
DR   SMART; SM01038; Bgal_small_N; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|ARBA:ARBA00022837};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001414}.
FT   DOMAIN          756..1086
FT                   /note="Beta galactosidase small chain/"
FT                   /evidence="ECO:0000259|SMART:SM01038"
SQ   SEQUENCE   1103 AA;  125876 MW;  AB3C82777C02EACE CRC64;
     MAEKTSDKPY WQDVQVVSVN KEYPRTSFMT YDNRTDALTG KFEKSNYYQL LNGTWKFFFA
     DSYKDLPANI TDPSVSTDSW NDIKVPGNWE VQGYGVAIYT NHGYEFKPRN PQPPSLPEAN
     PVGVYRRDID IPADWKERDI YLHLAGAKSG VYVYINGQEV GYSEDSKNPA EFLINKYVKP
     GKNVLTLKIF RWSTGSYLEC QDFWRISGIE RDVYLYSQPK AALKDFRITS TLDDSYKNGV
     FALNVDLRNH RPAATNLTLA YELLDAQGKV VATEEKTTYV PVNETRTLSF DKNLTDVHTW
     TSEHPNLYKL VMTVKEEGKV NEIIPFNVGF RRIEIKPIDQ KAANGKPYVC LFINGQPLKL
     KGVNIHEHNP ETGHYVTEEL MRRDFELMKQ HNLNSVRLCH YPQDRRFYEL CDEYGLYVYD
     EANIESHGMY YDLRKGGSLG NNPEWLKPHM DRTINMFERN KNYPSVTFWS LGNEAGNGYN
     FYQTYLWLKE ADKNIMQRPV NYERAQWEWN TDMYVPQYPG AGWLEDIGKN GSDRPIVPSE
     YAHAMGNSTG NLWGQWQAIY KYPNLQGGYI WDWVDQGLLQ KDKNGREYWA YGGDFGVNAP
     SDGNFLCNGL VNPDRGLHPA MAEVKYVHQN VGFDAIDAAS GKFNITNRFY FTNLKKYQIH
     YSVLANGKTV KSGKVSLDIA PQASKEFTVP VNGLKARPGT EYFVNFSVIA VEPEPLIPAG
     YEIAYDQFRL PVEAERNTYK ANGPALQTQT QGDELTVSSS KVNFVFNKKS GLVTSYKVDG
     TEYFKDGFGI QPNFWRAPND NDYGNGAPKR LHVWKQSSKD FHVTDTKVST EDKAVLLQAT
     YLLAAGNLYV VTYKIYPSGI VHVNAKFTST DMQAAETEVS EATRMATFTP GSDAARKAAS
     KLEVPRIGVR FRLPAQMNNV QYFGRGPEEN YIDRNHGTMV GIYQTTADKM YFNYVRPQEN
     GHHTDTRWLN LSPDKGNGLV IVADSLIGFN ALRNSVEDFD SEEALPHPYQ WNNFSPEEAA
     NHDEKAARNV LRRMHHVNDV TPRDFVEVCV DMKQQGVGGY DSWGARPEPF HQIPANREYN
     WGFTLVPVRS GSQATEAAKY DYR
//