Q89ZX0 (GLAB_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase B
EC=3.2.1.n1
EC=3.2.1.n2

Alternative name(s):
BtGal110B
Exo-alpha-galactosidase B
EC=3.2.1.22

Gene names

Name:	glaB
Ordered Locus Names:	BT_4251

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Ref.3 Hydrolysis of terminal, non-reducing linear (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Ref.3 Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. B subfamily. Contains 6 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 615	592	Alpha-1,3-galactosidase B		PRO_0000348478
Regions
	Repeat	282 – 313	32	PbH1 1
	Repeat	423 – 445	23	PbH1 2
	Repeat	446 – 467	22	PbH1 3
	Repeat	478 – 500	23	PbH1 4
	Repeat	520 – 541	22	PbH1 5
	Repeat	543 – 573	31	PbH1 6

Sequences

	Sequence		Length	Mass (Da)
	Q89ZX0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 19DB3A255E32510C	FASTA	615	69,373
	10 20 30 40 50 60 MRTFLSLKTC LLSALLLCVN SIAASKIISV SDFGLKPDSR INAVPFIQKA IDACKQHPGS 70 80 90 100 110 120 TLVFPKGRYD FWAQHAIEKD YYETNTYDVN PKILAVLLEQ INDLTIDGNG SEFIMHGRMQ 130 140 150 160 170 180 PFTLDHCRNI TLKNFSVDWE IPLTAQGIVT QSTSEYLEIE IDSHQYPYII ENKRLTFVGE 190 200 210 220 230 240 GWKSSLWAIM QFDPDTHLVL PNTGDNLGWR SYDATEINPG LIRLSDPKKE ADKFFPAPGT 250 260 270 280 290 300 VLVLRHSTRD HAGIFIYHSM DTKLENVKLF HTCGLGILSQ YSKNISFNDV HIIPNTSKKR 310 320 330 340 350 360 VLSGHDDGFH FMGCSGLLKI ENCSWAGLMD DPINIHGTCS RIMEVLSPTR IKCKFMQDMS 370 380 390 400 410 420 EGMEWGRPDE TIGFIEHKTM RTVATGKMNK FEALNKAEFI IELSVPLPAG VEAGYVIENL 430 440 450 460 470 480 TCTPDAEIRN CHFGSCRARG LLVSTPGKVI IENNVFESSG SAILIAGDAN AWYESGAVKD 490 500 510 520 530 540 VLIRNNDFRY PCNSSIYQFC EAVISIDPEI PTPEQKYPYH RNIRIMDNTF HLFDYPILFA 550 560 570 580 590 600 RSVNGLTFSS NTLIRDTTYQ PYHYRKEGIT LEACKSVVIS NNKIEGDVLG RIVTIEKMKP 610 SDVKISKNPF FKLKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacterial glycosidases for the production of universal red blood cells." Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H. Nat. Biotechnol. 25:454-464(2007) [PubMed: 17401360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.
[3]	"Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen." Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H. J. Biol. Chem. 283:8545-8554(2008) [PubMed: 18227066] [Abstract] Cited for: ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM109957 Genomic DNA. Translation: CAJ33353.1. AE015928 Genomic DNA. Translation: AAO79356.1.
RefSeq	NP_813162.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q89ZX0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1074743.
GenomeReviews	Gene locus BT_4251 in contig AE015928_GR.
KEGG	bth:BT_4251.
NMPDR	fig|226186.1.peg.4249.
PATRIC	21063550. VBIBacThe70966_4322.
Phylogenomic databases
HOGENOM	HBG345842.
OMA	LTWTPEV.
ProtClustDB	CLSK823424.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_4251-MONOMER.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 2 hits.
SMART	SM00710. PbH1. 6 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLAB_BACTN

Accession

Primary (citable) accession number: Q89ZX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program