Q89ZV6 (GUAA1_BACTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] 1
EC=6.3.5.2

Alternative name(s):
GMP synthetase 1
Glutamine amidotransferase 1

Gene names

Name:	guaA1
Ordered Locus Names:	BT_4265

Organism

Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomic identifier

226186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	GMP synthase [glutamine-hydrolyzing] 1 HAMAP MF_00344		PRO_0000140094
Regions
	Domain	4 – 193	190	Glutamine amidotransferase type-1
	Domain	194 – 382	189	GMPS ATP-PPase
	Nucleotide binding	221 – 227	7	ATP By similarity
Sites
	Active site	79	1	Nucleophile By similarity
	Active site	167	1	By similarity
	Active site	169	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89ZV6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8207C222AAA741F6	FASTA	507	56,656
	10 20 30 40 50 60 MQEKIIILDF GSQTTQLIGR RVRELDTYCE IVPYNKFPKE DPTIKGVILS GSPFSVYDKD 70 80 90 100 110 120 AFKVDLSEIR GKYPILGICY GAQFMAYTNN GKVEPAGTRE YGRAHLTSFC KDNVLFKGVR 130 140 150 160 170 180 ENTQVWMSHG DTITAIPDNF KKIASTDKVD IAAYQVEGEK VWGVQFHPEV FHSEDGTQIL 190 200 210 220 230 240 RNFVVDVCGC KQDWSPASFI ESTVAELKAQ LGDDKVVLGL SGGVDSSVAA VLLNRAIGKN 250 260 270 280 290 300 LTCIFVDHGM LRKNEFKNVM NDYECLGLNV IGVDASEKFF AELAGVTEPE RKRKIIGKGF 310 320 330 340 350 360 IDVFDVEAHK IKDVKWLAQG TIYPDCIESL SITGTVIKSH HNVGGLPEKM HLKLCEPLRL 370 380 390 400 410 420 LFKDEVRRVG RELGMPEHLI TRHPFPGPGL AVRILGDITR EKVRILQDAD DIYIQGLRDW 430 440 450 460 470 480 GLYDQVWQAG VILLPVQSVG VMGDERTYER AVALRAVTST DAMTADWAHL PYEFLGKISN 490 500 DIINKVKGVN RVTYDISSKP PATIEWE

References

[1]

"A genomic view of the human-Bacteroides thetaiotaomicron symbiosis." Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I. Science 299:2074-2076(2003) [PubMed: 12663928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015928 Genomic DNA. Translation: AAO79370.1.
RefSeq	NP_813176.1. NC_004663.1.
3D structure databases
ProteinModelPortal	Q89ZV6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1071739.
GenomeReviews	Gene locus BT_4265 in contig AE015928_GR.
KEGG	bth:BT_4265.
NMPDR	fig|226186.1.peg.4263.
PATRIC	21063584. VBIBacThe70966_4338.
Phylogenomic databases
HOGENOM	HBG391775.
OMA	YENAICL.
PhylomeDB	Q89ZV6.
ProtClustDB	PRK00074.
Enzyme and pathway databases
BioCyc	BTHE226186:BT_4265-MONOMER.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA1_BACTN

Accession

Primary (citable) accession number: Q89ZV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program