Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).By similarity

Miscellaneous

The active site is located at the dimer interface.By similarity

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.By similarity

Cofactori

a divalent metal cationBy similarityNote: Binds 1 divalent metal cation per subunit.By similarity

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Erythronate-4-phosphate dehydrogenase (pdxB)
  3. Phosphoserine aminotransferase (serC)
  4. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA)
  5. Pyridoxine 5'-phosphate synthase (pdxJ)
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138SubstrateBy similarity1
Binding sitei139SubstrateBy similarity1
Metal bindingi169Divalent metal cation; shared with dimeric partnerBy similarity1
Metal bindingi214Divalent metal cation; shared with dimeric partnerBy similarity1
Metal bindingi269Divalent metal cation; shared with dimeric partnerBy similarity1
Binding sitei277SubstrateBy similarity1
Binding sitei286SubstrateBy similarity1
Binding sitei295SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandMetal-binding, NAD, NADP

Enzyme and pathway databases

BioCyciBTHE:G13PU-9356-MONOMER
UniPathwayiUPA00244; UER00312

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseBy similarity (EC:1.1.1.262By similarity)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseBy similarity
Gene namesi
Name:pdxABy similarity
Ordered Locus Names:BT_4374
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000514911 – 3644-hydroxythreonine-4-phosphate dehydrogenaseAdd BLAST364

Proteomic databases

PaxDbiQ89ZK3
PRIDEiQ89ZK3

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi226186.BT_4374

Structurei

3D structure databases

ProteinModelPortaliQ89ZK3
SMRiQ89ZK3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.Curated

Phylogenomic databases

eggNOGiENOG4105CEZ Bacteria
COG1995 LUCA
HOGENOMiHOG000221591
InParanoidiQ89ZK3
KOiK00097
OMAiAPVHKGV
OrthoDBiPOG091H03XD

Family and domain databases

InterProiView protein in InterPro
IPR005255 PdxA_fam
PANTHERiPTHR30004 PTHR30004, 1 hit
PfamiView protein in Pfam
PF04166 PdxA, 1 hit
TIGRFAMsiTIGR00557 pdxA, 1 hit

Sequencei

Sequence statusi: Complete.

Q89ZK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEENKIRIGI TQGDINGVGY EVILKTFSDP TMLELCTPII YGSPKVAAYH
60 70 80 90 100
RKALDVQANF SIVNTASEAG YNRLSVVNCT DDEVKVEFSK PDPEAGKAAL
110 120 130 140 150
GALERAIEEY REGLIDVIVT APINKHTIQS EEFSFPGHTE YIEERLGNGN
160 170 180 190 200
KSLMILMKND FRVALVTTHI PVREIATTIT KELIQEKLMI FHRCLKQDFG
210 220 230 240 250
IGAPRIAVLS LNPHAGDGGL LGMEEQEIII PAMKEMEEKG IICYGPYAAD
260 270 280 290 300
GFMGSGNYTH FDGILAMYHD QGLAPFKALA MEDGVNYTAG LPVVRTSPAH
310 320 330 340 350
GTAYDIAGKG LASEDSFRQA IYVAIDVFRN RQREKAARVN PLRKQYYEKR
360
DDSDKLKLDT VDED
Length:364
Mass (Da):40,325
Last modified:June 1, 2003 - v1
Checksum:iFD60351E6D30985A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA Translation: AAO79479.1
RefSeqiNP_813285.1, NC_004663.1
WP_008760044.1, NC_004663.1

Genome annotation databases

EnsemblBacteriaiAAO79479; AAO79479; BT_4374
GeneIDi1071722
31618160
KEGGibth:BT_4374
PATRICifig|226186.12.peg.4451

Similar proteinsi

Entry informationi

Entry nameiPDXA_BACTN
AccessioniPrimary (citable) accession number: Q89ZK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health