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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 211ATP 1By similarity8
Nucleotide bindingi603 – 610ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHE:G13PU-9581-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:BT_4597
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001910921 – 862Chaperone protein ClpBAdd BLAST862

Proteomic databases

PaxDbiQ89YY3

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi226186.BT_4597

Structurei

3D structure databases

ProteinModelPortaliQ89YY3
SMRiQ89YY3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 141N-terminalBy similarityAdd BLAST141
Regioni157 – 338NBD1By similarityAdd BLAST182
Regioni339 – 543LinkerBy similarityAdd BLAST205
Regioni553 – 770NBD2By similarityAdd BLAST218
Regioni771 – 862C-terminalBy similarityAdd BLAST92

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili389 – 520By similarityAdd BLAST132

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z Bacteria
COG0542 LUCA
HOGENOMiHOG000218209
InParanoidiQ89YY3
KOiK03695
OMAiHHKVRIK
OrthoDBiPOG091H019M

Family and domain databases

Gene3Di1.10.1780.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR017730 Chaperonin_ClpB
IPR019489 Clp_ATPase_C
IPR004176 Clp_N
IPR036628 Clp_N_dom_sf
IPR001270 ClpA/B
IPR018368 ClpA/B_CS1
IPR028299 ClpA/B_CS2
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF07724 AAA_2, 1 hit
PF02861 Clp_N, 2 hits
PF10431 ClpB_D2-small, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01086 ClpB_D2-small, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF81923 SSF81923, 1 hit
TIGRFAMsiTIGR03346 chaperone_ClpB, 1 hit
PROSITEiView protein in PROSITE
PS00870 CLPAB_1, 1 hit
PS00871 CLPAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q89YY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFNNFTIKS QEAVQEAVNL VQSRGQQAIE PAHILHGVMK VGENVTNFIF
60 70 80 90 100
QKLGMNGQQV ALVVDKQIES LPKVSGGEPY LSRESNEVLQ KATQYSKEMG
110 120 130 140 150
DEFVSLEHIL LALLTVKSTV STILKDAGMT EKELRNAINE LRKGEKVTSQ
160 170 180 190 200
SSEDNYQSLE KYAINLNEAA RSGKLDPVIG RDEEIRRVLQ ILSRRTKNNP
210 220 230 240 250
ILIGEPGTGK TAIVEGLAHR ILRGDVPENL KNKQVYSLDM GALVAGAKYK
260 270 280 290 300
GEFEERLKSV VNEVKKSEGD IILFIDEIHT LVGAGKGEGA MDAANILKPA
310 320 330 340 350
LARGELRSIG ATTLDEYQKY FEKDKALERR FQIVQVDEPD TLSTISILRG
360 370 380 390 400
LKERYENHHH VRIKDDAIIA AVELSSRYIT DRFLPDKAID LMDEAAAKLR
410 420 430 440 450
MEVDSVPEEL DEISRKIKQL EIEREAIKRE NDKPKLEIIG KELAELKEVE
460 470 480 490 500
KSFKAKWQSE KTLMDKIQQN KVEIENLKFE AEKAEREGDY GKVAEIRYGK
510 520 530 540 550
LQALDKEIED TQEKLRGMQG DKAMIKEEVD AEDIADVVSR WTGIPVSKMM
560 570 580 590 600
QSEKDKLLHL EDELHQRVIG QDEAIEAVAD AVRRSRAGLQ DPKRPIGSFI
610 620 630 640 650
FLGTTGVGKT ELAKALAEFL FDDESMMTRI DMSEYQEKHS VSRLVGAPPG
660 670 680 690 700
YVGYDEGGQL TEAIRRKPYS VVLFDEIEKA HPDVFNILLQ VLDDGRLTDN
710 720 730 740 750
KGRVVNFKNT IIIMTSNMGS SYIQSQMEKL SGSNKEEVIE ETKKEVMNML
760 770 780 790 800
KKNIRPEFLN RIDETIMFLP LTETEIRQIV LLQIKGVQKM LAENGVELEM
810 820 830 840 850
TDAALNFLSQ VGYDPEFGAR PVKRAIQRYL LNDLSKKLLS QEVDRSKAII
860
VDSNGDGLVF RN
Length:862
Mass (Da):97,189
Last modified:June 1, 2003 - v1
Checksum:iFD5EC4B9B236D3DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA Translation: AAO79702.1
RefSeqiNP_813508.1, NC_004663.1
WP_008764749.1, NC_004663.1

Genome annotation databases

EnsemblBacteriaiAAO79702; AAO79702; BT_4597
GeneIDi1073545
31617831
KEGGibth:BT_4597
PATRICifig|226186.12.peg.4676

Similar proteinsi

Entry informationi

Entry nameiCLPB_BACTN
AccessioniPrimary (citable) accession number: Q89YY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health