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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi204 – 2118ATP 1By similarity
Nucleotide bindingi603 – 6108ATP 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-4679-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:BT_4597
OrganismiBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Taxonomic identifieri226186 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000001414 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Chaperone protein ClpBPRO_0000191092Add
BLAST

Proteomic databases

PaxDbiQ89YY3.

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi226186.BT_4597.

Structurei

3D structure databases

ProteinModelPortaliQ89YY3.
SMRiQ89YY3. Positions 157-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 141141N-terminalBy similarityAdd
BLAST
Regioni157 – 338182NBD1By similarityAdd
BLAST
Regioni339 – 543205LinkerBy similarityAdd
BLAST
Regioni553 – 770218NBD2By similarityAdd
BLAST
Regioni771 – 86292C-terminalBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili389 – 520132By similarityAdd
BLAST

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218209.
InParanoidiQ89YY3.
KOiK03695.
OMAiPRAKQIF.
OrthoDBiEOG65F8SM.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89YY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFNNFTIKS QEAVQEAVNL VQSRGQQAIE PAHILHGVMK VGENVTNFIF
60 70 80 90 100
QKLGMNGQQV ALVVDKQIES LPKVSGGEPY LSRESNEVLQ KATQYSKEMG
110 120 130 140 150
DEFVSLEHIL LALLTVKSTV STILKDAGMT EKELRNAINE LRKGEKVTSQ
160 170 180 190 200
SSEDNYQSLE KYAINLNEAA RSGKLDPVIG RDEEIRRVLQ ILSRRTKNNP
210 220 230 240 250
ILIGEPGTGK TAIVEGLAHR ILRGDVPENL KNKQVYSLDM GALVAGAKYK
260 270 280 290 300
GEFEERLKSV VNEVKKSEGD IILFIDEIHT LVGAGKGEGA MDAANILKPA
310 320 330 340 350
LARGELRSIG ATTLDEYQKY FEKDKALERR FQIVQVDEPD TLSTISILRG
360 370 380 390 400
LKERYENHHH VRIKDDAIIA AVELSSRYIT DRFLPDKAID LMDEAAAKLR
410 420 430 440 450
MEVDSVPEEL DEISRKIKQL EIEREAIKRE NDKPKLEIIG KELAELKEVE
460 470 480 490 500
KSFKAKWQSE KTLMDKIQQN KVEIENLKFE AEKAEREGDY GKVAEIRYGK
510 520 530 540 550
LQALDKEIED TQEKLRGMQG DKAMIKEEVD AEDIADVVSR WTGIPVSKMM
560 570 580 590 600
QSEKDKLLHL EDELHQRVIG QDEAIEAVAD AVRRSRAGLQ DPKRPIGSFI
610 620 630 640 650
FLGTTGVGKT ELAKALAEFL FDDESMMTRI DMSEYQEKHS VSRLVGAPPG
660 670 680 690 700
YVGYDEGGQL TEAIRRKPYS VVLFDEIEKA HPDVFNILLQ VLDDGRLTDN
710 720 730 740 750
KGRVVNFKNT IIIMTSNMGS SYIQSQMEKL SGSNKEEVIE ETKKEVMNML
760 770 780 790 800
KKNIRPEFLN RIDETIMFLP LTETEIRQIV LLQIKGVQKM LAENGVELEM
810 820 830 840 850
TDAALNFLSQ VGYDPEFGAR PVKRAIQRYL LNDLSKKLLS QEVDRSKAII
860
VDSNGDGLVF RN
Length:862
Mass (Da):97,189
Last modified:June 1, 2003 - v1
Checksum:iFD5EC4B9B236D3DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO79702.1.
RefSeqiNP_813508.1. NC_004663.1.
WP_008764749.1. NC_004663.1.

Genome annotation databases

EnsemblBacteriaiAAO79702; AAO79702; BT_4597.
GeneIDi1073545.
KEGGibth:BT_4597.
PATRICi21064264. VBIBacThe70966_4676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015928 Genomic DNA. Translation: AAO79702.1.
RefSeqiNP_813508.1. NC_004663.1.
WP_008764749.1. NC_004663.1.

3D structure databases

ProteinModelPortaliQ89YY3.
SMRiQ89YY3. Positions 157-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226186.BT_4597.

Proteomic databases

PaxDbiQ89YY3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO79702; AAO79702; BT_4597.
GeneIDi1073545.
KEGGibth:BT_4597.
PATRICi21064264. VBIBacThe70966_4676.

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218209.
InParanoidiQ89YY3.
KOiK03695.
OMAiPRAKQIF.
OrthoDBiEOG65F8SM.

Enzyme and pathway databases

BioCyciBTHE226186:GJXV-4679-MONOMER.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis."
    Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C., Hooper L.V., Gordon J.I.
    Science 299:2074-2076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482.

Entry informationi

Entry nameiCLPB_BACTN
AccessioniPrimary (citable) accession number: Q89YY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: January 20, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.