Reviewed, UniProtKB/Swiss-Prot Q89XW8 (AROB_BRAJA)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate synthase
    EC=4.2.3.4

Gene names

Name:	aroB
Ordered Locus Names:	bll0187

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate. HAMAP MF_00110
Cofactor	NAD By similarity. HAMAP MF_00110 Divalent metal cations By similarity. HAMAP MF_00110
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7. HAMAP MF_00110
Subunit structure	Monomer By similarity. HAMAP MF_00110
Subcellular location	Cytoplasm Probable HAMAP MF_00110.
Sequence similarities	Belongs to the dehydroquinate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-dehydroquinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	3-dehydroquinate synthase HAMAP MF_00110		PRO_0000140714

Sequences

	Sequence		Length	Mass (Da)
	Q89XW8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 13189D8699216EE8	FASTA	382	40,277
	10 20 30 40 50 60 MTAPLKHSDP VNVDVALGDR AYDIVIGRGV LASLGERVAA LRPGVRTAVV TDRTVAKHWL 70 80 90 100 110 120 EPTEASLAAA GIPTSRIVVE EGEISKTYAG LEKVSEALIA AKIERNDLVI ALGGGVVGDL 130 140 150 160 170 180 AGFAAAILRR GVDFVQVPTS LLAQVDSSVG GKTGINSPQG KNLLGAFHQP VLVIADTAVL 190 200 210 220 230 240 DTLSPRQFHA GYAEVAKYGV LGDEAFFTWL EKNHSDIFKG GSAREHAIAT SCRAKAGIVS 250 260 270 280 290 300 RDERETGERA LLNLGHTFGH ALEAATGFSD RLFHGEGVAI GMTLAAQFSA KLGMIGEADA 310 320 330 340 350 360 ARVERHLIEA GLPTRLQDIA GFSQEGLADA DALMALMAQD KKVKRGKLTF ILLEAVGRAV 370 380 IAKDVEPAPV RDFLKEKLAQ KA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC45452.1.
RefSeq	NP_766827.1.
3D structure databases
SMR	Q89XW8. Positions 17-373.
ModBase	Search...
Genome annotation databases
GeneID	1049146.
GenomeReviews	Gene locus bll0187 in contig BA000040_GR.
KEGG	bja:bll0187.
NMPDR	fig|224911.1.peg.187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG632303.
OMA	APESMDF.
PhylomeDB	Q89XW8.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0187-MONOMER.
BRENDA	4.2.3.4. 280.
Family and domain databases
HAMAP	MF_00110. DHQ_synthase. [Tree]
InterPro	IPR016303. DHQ_synth. IPR002658. DHQ_synth_AroB. IPR016037. DHQ_synth_AroB_sub. [Graphical view]
PANTHER	PTHR21090:SF1. DHQ_synth_AroB. 1 hit.
Pfam	PF01761. DHQ_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001455. DHQ_synth. 1 hit.
TIGRFAMs	TIGR01357. aroB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROB_BRAJA

Accession

Primary (citable) accession number: Q89XW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)