Q89XU2 (UBIG_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-demethylubiquinone-9 3-methyltransferase
EC=2.1.1.64

Alternative name(s):
2-polyprenyl-6-hydroxyphenyl methylase
EC=2.1.1.222
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase
Short name=DHHB methyltransferase

Gene names

Name:	ubiG
Ordered Locus Names:	bll0214

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-specific O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway By similarity.
Catalytic activity	S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n. HAMAP MF_00472 S-adenosyl-L-methionine + 3-(all-trans-polyprenyl)benzene-1,2-diol = S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-polyprenyl)phenol.
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP MF_00472
Sequence similarities	Belongs to the methyltransferase superfamily. UbiG/COQ3 family.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ubiquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Inferred from electronic annotation. Source: InterPro 3-demethylubiquinone-9 3-O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	3-demethylubiquinone-9 3-methyltransferase HAMAP MF_00472		PRO_0000193371

Sequences

	Sequence		Length	Mass (Da)
	Q89XU2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C834A007A638F774	FASTA	253	28,120
	10 20 30 40 50 60 MQQNTSATAQ PGSTVDAAEI AKFSKLSAEW WDPKGKMAPL HRINPLRLGY IRDAACRKFE 70 80 90 100 110 120 RNVRSLNCLG GLRVLDIGCG AGLLCEPLSR LGAQVIGVDP SQSNIAAAKL HADKSHLAID 130 140 150 160 170 180 YRCTTVEEID PRERFDIVLA MEVVEHVVDV GVFLKRCAAM LKPNGLMVVS TLNRNWKSFA 190 200 210 220 230 240 LAIVGAEYVL RWLPRGTHEW NKFVTPDELT KYLLDNRLVI TEQTGVVYSP FADKWTLSSD 250 MDVNYMVVAE GMV

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC45479.1.
RefSeq	NP_766854.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89XU2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1054073.
GenomeReviews	Gene locus bll0214 in contig BA000040_GR.
KEGG	bja:bll0214.
NMPDR	fig|224911.1.peg.214.
PATRIC	21183857. VBIBraJap65052_0208.
Phylogenomic databases
HOGENOM	HBG738842.
OMA	KFITPDE.
PhylomeDB	Q89XU2.
ProtClustDB	PRK05134.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0214-MONOMER.
Family and domain databases
HAMAP	MF_00472. UbiG. [Tree]
InterPro	IPR013216. Methyltransf_11. IPR010233. UbiG_MeTrfase. [Graphical view]
KO	K00568.
PANTHER	PTHR23134. PTHR23134. 1 hit.
Pfam	PF08241. Methyltransf_11. 1 hit. [Graphical view]
TIGRFAMs	TIGR01983. UbiG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UBIG_BRAJA

Accession

Primary (citable) accession number: Q89XU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program