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Reviewed, UniProtKB/Swiss-Prot Q89XT5 (GLO2_BRAJA)

Last modified February 9, 2010. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Hydroxyacylglutathione hydrolase
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II
      Short name=Glx II
Gene names
Name: gloB
Ordered Locus Names: blr0222
OrganismBradyrhizobium japonicum [Complete proteome] [HAMAP]
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

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Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374

Catalytic activity

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374

Subunit structure

Monomer By similarity. HAMAP MF_01374

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

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Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionhydroxyacylglutathione hydrolase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 255255Hydroxyacylglutathione hydrolase HAMAP MF_01374
PRO_0000309628

Sites

Metal binding561Zinc 1 By similarity
Metal binding581Zinc 1 By similarity
Metal binding601Zinc 2 By similarity
Metal binding611Zinc 2 By similarity
Metal binding1141Zinc 1 By similarity
Metal binding1331Zinc 1 By similarity
Metal binding1331Zinc 2 By similarity
Metal binding1711Zinc 2 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q89XT5-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: EBCC3C87ACB73A18

FASTA25527,962
        10         20         30         40         50         60 
MAAEIRTFSC LNDNFGYLIH DVETKATASI DAPEAGPILK ALEREGWQLT DILITHHHGD 

        70         80         90        100        110        120 
HVGGVAELKH KYNCRVVAPH DKTTAIANVD LRVANADVVK VGNLLARVVE TPGHTLDHIS 

       130        140        150        160        170        180 
YVFDTEKTVF AADTLFSIGC GRVFEGTYPM MWDSLLKLRA LPDDFKLYCG HEYTASNVKF 

       190        200        210        220        230        240 
ALTVDPDNAA LQARAAEVAK LRAENKPTIP SLLGDEKRAN VFLRADDPSV AARLHMKGAD 

       250 
AAAVFGELRE RKNKS

« Hide

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References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000040 Genomic DNA. Translation: BAC45487.1.
RefSeqNP_766862.1.

3D structure databases

HSSPHSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
SMRQ89XT5. Positions 4-254.
ModBaseSearch...

Genome annotation databases

GeneID1050902.
GenomeReviewsGene locus blr0222 in contig BA000040_GR.
KEGGbja:blr0222.
NMPDRfig|224911.1.peg.222.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG753931.
OMAFGHEYTL.
PhylomeDBQ89XT5.

Enzyme and pathway databases

BioCycBJAP224911:BLR0222-MONOMER.
BRENDA3.1.2.6. 280.

Family and domain databases

HAMAPMF_01374. Glyoxalase_2.
[Tree]
InterProIPR001279. Blactmase-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
TIGRFAMsTIGR03413. GSH_gloB. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameGLO2_BRAJA
AccessionPrimary (citable) accession number: Q89XT5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 2003
Last modified: February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents