Reviewed, UniProtKB/Swiss-Prot Q89XM2 (FUMC2_BRAJA)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II 2
      Short name=Fumarase C 2
    EC=4.2.1.2

Gene names

Name:	fumC2
Ordered Locus Names:	bll0286

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Fumarate hydratase class II 2 HAMAP MF_00743		PRO_0000161260
Regions
	Region	129 – 132	4	B site By similarity
	Region	139 – 141	3	Substrate binding By similarity
Sites
	Binding site	100	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89XM2-1 [UniParc]. Last modified December 15, 2003. Version 2. Checksum: A33EC007B6A88B44	FASTA	478	51,299
	10 20 30 40 50 60 MNDLRKETDS LGEVNVPADK LWGAQTQRSL EHFSIGRDLM PREMIQSYAI LKKAAANANY 70 80 90 100 110 120 AGGRLDGKIH KLIVHTCDEI LAGQHHDMFP LHVWMTGSGT QFNMNVNEVI SNRCCQLAGT 130 140 150 160 170 180 ALGSKLPVHP NDHVNMSQSS NDSFPSAMYI AAAMNVTQRL VPAVEALHDA IAAKSNQWDD 190 200 210 220 230 240 IVKIGRTHMQ DATPLTLGQE WSGYAAMLAD GLARIDDALK GVFRLALGGT AVGTGINAAP 250 260 270 280 290 300 GFAEAVAAEI ARLTGLPFVS APNKFAVQGA HDALVQLSGT LRTLAGSLYK IANDIRLLSC 310 320 330 340 350 360 GPRAGFAELR IPENEPGSSI MPGKVNPTQA EALTMIAVQV MANDVAVGFG GAGGYLEMNV 370 380 390 400 410 420 YKPLIIHNIA QSVTILTDGC TNFRTFLVEG TEPNRKKIKE YVERSLMLVT ALAPVIGYDK 430 440 450 460 470 ASRIAHYAMD NDLTLKSAAL KLGFVTEPEF DRIVDPAKMV KPYVAEIKVP LAIGAKTG

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC45551.1. Different initiation.
RefSeq	NP_766926.2.
3D structure databases
HSSP	HSSP built from PDB template 1FUR based on UniProtKB P05042.
ModBase	Search...
Genome annotation databases
GeneID	1048934.
GenomeReviews	Gene locus bll0286 in contig BA000040_GR.
KEGG	bja:bll0286.
NMPDR	fig|224911.1.peg.286.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89XM2.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0286-MON.
BRENDA	4.2.1.2. 280.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC2_BRAJA

Accession

Primary (citable) accession number: Q89XM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	September 22, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)