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Q89XJ6 (NOSZ_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitrous-oxide reductase
EC=1.7.2.4
Alternative name(s):
N(2)OR
N2O reductase
Gene names
Name:nosZ
Ordered Locus Names:blr0315
OrganismBradyrhizobium japonicum
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length650 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide By similarity. HAMAP MF_00716

Catalytic activity

Nitrogen + H2O + 2 cytochrome c = nitrous oxide + 2 reduced cytochrome c. HAMAP MF_00716

Cofactor

Binds 2 calcium ions per subunit By similarity. HAMAP MF_00716

Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4. HAMAP MF_00716

Subunit structure

Homodimer By similarity. HAMAP MF_00716

Subcellular location

Periplasm By similarity HAMAP MF_00716.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_00716

Sequence similarities

Belongs to the nosZ family.

In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4646Tat-type signal Potential
Chain47 – 650604Nitrous-oxide reductase HAMAP MF_00716
PRO_0000019824

Regions

Region552 – 65099COX2-like HAMAP MF_00716

Sites

Metal binding1471Copper Z2 By similarity
Metal binding1481Copper Z3 By similarity
Metal binding1961Copper Z2 By similarity
Metal binding2731Calcium 2; via carbonyl oxygen By similarity
Metal binding2761Calcium 2 By similarity
Metal binding2841Calcium 2; via carbonyl oxygen By similarity
Metal binding2901Calcium 2 By similarity
Metal binding3351Calcium 2 By similarity
Metal binding3371Copper Z1 By similarity
Metal binding3921Copper Z1 By similarity
Metal binding4431Copper Z3 By similarity
Metal binding4641Calcium 1; via carbonyl oxygen By similarity
Metal binding4791Calcium 1 By similarity
Metal binding5041Copper Z4 By similarity
Metal binding5931Copper A1 By similarity
Metal binding6281Copper A1 By similarity
Metal binding6281Copper A2 By similarity
Metal binding6301Copper A2; via carbonyl oxygen By similarity
Metal binding6321Copper A1 By similarity
Metal binding6321Copper A2 By similarity
Metal binding6361Copper A2 By similarity
Metal binding6391Copper A1 By similarity

Experimental info

Sequence conflict3671D → N in CAA05518. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q89XJ6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 1B6C547D90501C5B

FASTA65071,674
        10         20         30         40         50         60 
MSDSDNIKGV SRRTLLGTTA AAAGVGLAGG AVVTKDGAGF VSTADAQTKS AAPKAPPARP 

        70         80         90        100        110        120 
AVQKTEVAPG ELDEYYVFFS SGQSGEMRII GLPSMRELMR VPVFNRCSAT GWGQTNESLK 

       130        140        150        160        170        180 
VLTEGMQPAT REFLKNRGGT FMNGDLHHPH VSFTDGTYDG RYAFMNDKAN SRVARVRLDV 

       190        200        210        220        230        240 
MKCDKIIELP NQHTVHGLRL QKYPRTGYVF CNGEDGVPLP NDGKVLDNPK EYHSIFTALD 

       250        260        270        280        290        300 
GDTMKVAWQV MVSGNLDNVD SDYQGKYCFS TCYNAEEGVT LAEMTANEQD WVVIFNLKRI 

       310        320        330        340        350        360 
EEAVKKGDFK EMNGVPVIDG RKGSPYTRYV PVSNNPHGMN TAPDGIHIVA AGKLSPTVTV 

       370        380        390        400        410        420 
MDVRLFDQLF DDKIKPRDVV VAEPELGLGP LHTAYDGKGN AYTTLFLDSQ VVKWNIDLAK 

       430        440        450        460        470        480 
RAFKGEKVDP IIQKLDVHYQ PGHNHSSMGQ TKEADGKWLI SLNKFSKDRF LNVGPLKPEN 

       490        500        510        520        530        540 
DQLIDISGDQ MKLVHDGPSF AEPHDATIVH RSKINPISIW DRADPMFADA VKQAKADGIN 

       550        560        570        580        590        600 
LEADSKIIRD GNKVRVYMTS TAPAFGLEQF QVKQGDQVTV YITNIDAVED LTHGFCIVNY 

       610        620        630        640        650 
GIQMEVAPMA TASVSFSADK AGVYWYYCSW FCHAMHMEMK GRMFVEPKSV

« Hide

References

« Hide 'large scale' references
[1]"Bradyrhizobium japonicum nosRZDFYLX gene cluster."
Bedmar E.J., Velasco L., Xu C.A., Delgado M.J.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: USDA 110.
[2]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ002531 Genomic DNA. Translation: CAA05518.1.
BA000040 Genomic DNA. Translation: BAC45580.1.
PIRT44660.
RefSeqNP_766955.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89XJ6.
SMRQ89XJ6. Positions 67-648.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1052167.
GenomeReviewsGene locus blr0315 in contig BA000040_GR.
KEGGbja:blr0315.
NMPDRfig|224911.1.peg.315.
PATRIC21184065. VBIBraJap65052_0312.

Phylogenomic databases

HOGENOMHBG308574.
OMAKLVHDGP.
PhylomeDBQ89XJ6.
ProtClustDBPRK02888.

Enzyme and pathway databases

BioCycBJAP224911:BLR0315-MONOMER.
BRENDA1.7.99.6. 929.

Family and domain databases

HAMAPMF_00716. NosZ.
[Tree]
InterProIPR008972. Cupredoxin.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011045. N2O_reductase_N.
IPR023644. NO_Rdtase.
IPR006311. TAT_signal.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 1 hit.
G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KOK00376.
PfamPF00116. COX2. 1 hit.
[Graphical view]
SUPFAMSSF49503. Cupredoxin. 1 hit.
SSF50974. N2O_reductase_N. 1 hit.
PROSITEPS00078. COX2. False negative.
PS50857. COX2_CUA. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOSZ_BRAJA
AccessionPrimary (citable) accession number: Q89XJ6
Secondary accession number(s): O31382
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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