Q89XH1 (HGD_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	bll0343

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium japonicum

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.
Sequence caution	The sequence BAC45608.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_0000220246
Sites
	Metal binding	346	1	Iron By similarity
	Metal binding	352	1	Iron By similarity
	Metal binding	382	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89XH1 [UniParc]. Last modified June 6, 2003. Version 1. Checksum: F6DD465E68735D3C	FASTA	448	49,780
	10 20 30 40 50 60 MNINTSPDQI IRSSAQVTPG YMSGFGNSFE TEALPGALPI GRNSPQRCAY GLYAEQLSGS 70 80 90 100 110 120 PFTAPRGTNE RSWLYRIRPS VKHSGRFEKA DAGLWRSAPC HEYDLPIAQM RWDPTPVPKE 130 140 150 160 170 180 EVTFVQGVQT MTTAGDVNTQ AGMAAHVYLI TKSMVDQHFY NADGELMFVL QQGNLRLVTE 190 200 210 220 230 240 FGRIDAEPGE IVVIPRGVKF RVEIPNGPAR GYLCENYGGA FTLPERGPIG ANCLANARDF 250 260 270 280 290 300 LTPVANYEDK DTPTELFVKW GGSLFKTTLP HSPIDVVAWH GNYAPYKYDL RTFSPVGAIG 310 320 330 340 350 360 FDHPDPSIFT VLTSPSETAG TANIDFVIFP ERWMVADNTF RPPWYHMNIM SEFMGLIYGV 370 380 390 400 410 420 YDAKPQGFVP GGMSLHNCML PHGPDRDAFE HASNGELKPV KLTGTMAFMF ETRYPQRVTA 430 440 HAANASTLQD DYADCWKGLE KRFDPNRP

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC45608.1. Different initiation.
RefSeq	NP_766983.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89XH1.
ModBase	Search...
Protein-protein interaction databases
STRING	224911.bll0343.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC45608; BAC45608; BAC45608.
GeneID	1053162.
KEGG	bja:bll0343.
PATRIC	21184127. VBIBraJap65052_0342.
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-345-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 3 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_BRAJA

Accession

Primary (citable) accession number: Q89XH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 6, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program