Reviewed, UniProtKB/Swiss-Prot Q89XA0 (LEU31_BRAJA)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase 1
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase 1
      Short name=IMDH 1
    3-IPM-DH 1

Gene names

Name:	leuB1
Ordered Locus Names:	bll0414

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	3-isopropylmalate dehydrogenase 1		PRO_0000250104
Regions
	Nucleotide binding	293 – 305	13	NAD By similarity
Sites
	Metal binding	230	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Metal binding	258	1	Magnesium or manganese By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity
	Site	146	1	Important for catalysis By similarity
	Site	197	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89XA0-1 [UniParc]. Last modified September 19, 2006. Version 2. Checksum: 0B418C546FC8D01C	FASTA	379	40,723
	10 20 30 40 50 60 MNTLSNTITV AVVGGEGIGP EVTDQSHRIL KWFSDRRGAP VILREAQYGL IPYLATGKVL 70 80 90 100 110 120 PDDTVEAMEE ADAILWGATG GPETTEVPPA ARKAGSLLSL RSKYDLYANL RPIVANPALA 130 140 150 160 170 180 DSAPLKAAVL KDVDFIIIRE LTSGIYFGEP RGIETLPDGQ RRGFNTQQYT TSQIRRVART 190 200 210 220 230 240 AFELARTRKG RVCSVDKANV LETSVLWREE VTALHEAEFS DVELTHLYVD NAAMQIVRAP 250 260 270 280 290 300 SQFDVMVTCN IFGDILSDCA AMASGSLGML PSVSLGPPDR LGRRKALYEP VHGSAPDIAG 310 320 330 340 350 360 KGIANPLGSI LSVAMMLRIT LHRPEDAALL EKAVDTALAA GARTADIAEP GAKRLSTQEM 370 GDAVLNALDK VVGKEREHA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC45679.1. Different initiation.
RefSeq	NP_767054.1.
3D structure databases
HSSP	HSSP built from PDB template 1A05 based on UniProtKB Q56268.
ModBase	Search...
Genome annotation databases
GeneID	1054690.
GenomeReviews	Gene locus bll0414 in contig BA000040_GR.
KEGG	bja:bll0414.
NMPDR	fig|224911.1.peg.414.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89XA0.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0414-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU31_BRAJA

Accession

Primary (citable) accession number: Q89XA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	September 19, 2006
Last modified:	November 4, 2008
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)