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Q89X86

- PROB_BRADU

UniProt

Q89X86 - PROB_BRADU

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Protein
Glutamate 5-kinase
Gene
proB, blr0428
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP By similarity
Binding sitei56 – 561Substrate By similarity
Binding sitei143 – 1431Substrate By similarity
Binding sitei155 – 1551Substrate; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi175 – 1762ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. glutamate 5-kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-431-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinase (EC:2.7.2.11)
Alternative name(s):
Gamma-glutamyl kinase
Short name:
GK
Gene namesi
Name:proB
Ordered Locus Names:blr0428
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Glutamate 5-kinaseUniRule annotation
PRO_0000109650Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.blr0428.

Structurei

3D structure databases

ProteinModelPortaliQ89X86.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini281 – 35878PUA
Add
BLAST

Sequence similaritiesi

Contains 1 PUA domain.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OrthoDBiEOG6PGK7G.
PhylomeDBiQ89X86.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89X86-1 [UniParc]FASTAAdd to Basket

« Hide

MASPELSQFR RIVVKVGSAL LVDSDKGEVR TSWLAALADD MAKLHKEGRD    50
VLVVSSGSIA LGRSRLKLPR GPLKLEESQA AAAVGQIALA RIWSEVLGAH 100
GIDAGQILVT LQDTEERRRY LNARSTIGKL LEWRAIPVIN ENDTVATTEI 150
RYGDNDRLAA RVATMASADL LVLLSDIDGL YDAPPKNNPN AKLIPVVDSI 200
SSEIEAVAGD AESELSRGGM RTKVEAAKIA TTGGTHMLIA SGKIEHPLQA 250
IANGGRCTWF LTPANPITSR KRWIAGTLEP KGTLTIDAGA VTALRAGASL 300
LPAGVIKVEG QFARGDAVIV RGPDTSEIGR GLIAYDADDA ERIKGRSSPD 350
VMTILGISGR AEMIHRDDLV VGG 373
Length:373
Mass (Da):39,540
Last modified:June 1, 2003 - v1
Checksum:i46076246031B8332
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC45693.1.
RefSeqiNP_767068.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC45693; BAC45693; BAC45693.
GeneIDi1054409.
KEGGibja:blr0428.
PATRICi21184317. VBIBraJap65052_0436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC45693.1 .
RefSeqi NP_767068.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89X86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr0428.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC45693 ; BAC45693 ; BAC45693 .
GeneIDi 1054409.
KEGGi bja:blr0428.
PATRICi 21184317. VBIBraJap65052_0436.

Phylogenomic databases

eggNOGi COG0263.
HOGENOMi HOG000246368.
KOi K00931.
OrthoDBi EOG6PGK7G.
PhylomeDBi Q89X86.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
BioCyci BJAP224911:GJEJ-431-MONOMER.

Family and domain databases

Gene3Di 2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPi MF_00456. ProB.
InterProi IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view ]
PIRSFi PIRSF000729. GK. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SMARTi SM00359. PUA. 1 hit.
[Graphical view ]
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsi TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiPROB_BRADU
AccessioniPrimary (citable) accession number: Q89X86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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