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Q89X86

- PROB_BRADU

UniProt

Q89X86 - PROB_BRADU

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Protein

Glutamate 5-kinase

Gene

proB

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATPUniRule annotation
Binding sitei56 – 561SubstrateUniRule annotation
Binding sitei143 – 1431SubstrateUniRule annotation
Binding sitei155 – 1551Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi175 – 1762ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: InterPro

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-431-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:blr0428
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Glutamate 5-kinasePRO_0000109650Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.blr0428.

Structurei

3D structure databases

ProteinModelPortaliQ89X86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini281 – 35878PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
InParanoidiQ89X86.
KOiK00931.
OrthoDBiEOG6PGK7G.
PhylomeDBiQ89X86.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89X86-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPELSQFR RIVVKVGSAL LVDSDKGEVR TSWLAALADD MAKLHKEGRD
60 70 80 90 100
VLVVSSGSIA LGRSRLKLPR GPLKLEESQA AAAVGQIALA RIWSEVLGAH
110 120 130 140 150
GIDAGQILVT LQDTEERRRY LNARSTIGKL LEWRAIPVIN ENDTVATTEI
160 170 180 190 200
RYGDNDRLAA RVATMASADL LVLLSDIDGL YDAPPKNNPN AKLIPVVDSI
210 220 230 240 250
SSEIEAVAGD AESELSRGGM RTKVEAAKIA TTGGTHMLIA SGKIEHPLQA
260 270 280 290 300
IANGGRCTWF LTPANPITSR KRWIAGTLEP KGTLTIDAGA VTALRAGASL
310 320 330 340 350
LPAGVIKVEG QFARGDAVIV RGPDTSEIGR GLIAYDADDA ERIKGRSSPD
360 370
VMTILGISGR AEMIHRDDLV VGG
Length:373
Mass (Da):39,540
Last modified:June 1, 2003 - v1
Checksum:i46076246031B8332
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC45693.1.
RefSeqiNP_767068.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC45693; BAC45693; BAC45693.
GeneIDi1054409.
KEGGibja:blr0428.
PATRICi21184317. VBIBraJap65052_0436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC45693.1 .
RefSeqi NP_767068.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89X86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr0428.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC45693 ; BAC45693 ; BAC45693 .
GeneIDi 1054409.
KEGGi bja:blr0428.
PATRICi 21184317. VBIBraJap65052_0436.

Phylogenomic databases

eggNOGi COG0263.
HOGENOMi HOG000246368.
InParanoidi Q89X86.
KOi K00931.
OrthoDBi EOG6PGK7G.
PhylomeDBi Q89X86.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00359 .
BioCyci BJAP224911:GJEJ-431-MONOMER.

Family and domain databases

Gene3Di 2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPi MF_00456. ProB.
InterProi IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view ]
PIRSFi PIRSF000729. GK. 1 hit.
PRINTSi PR00474. GLU5KINASE.
SMARTi SM00359. PUA. 1 hit.
[Graphical view ]
SUPFAMi SSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsi TIGR01027. proB. 1 hit.
PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiPROB_BRADU
AccessioniPrimary (citable) accession number: Q89X86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3