Reviewed, UniProtKB/Swiss-Prot Q89X85 (PROA_BRAJA)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	blr0429

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189704

Sequences

	Sequence		Length	Mass (Da)
	Q89X85-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C043AA17C4682B50	FASTA	435	45,636
	10 20 30 40 50 60 MAAPLKAVDG NADRTGDLQA LMSDLAARAR AAARVLALAP PEQKNRALEA MERAIRSNAA 70 80 90 100 110 120 AILAANAEDV AEARASSNMT ASFIDRLTLT PARVESMAEG IGIVRGIADP VGIVTESWQR 130 140 150 160 170 180 PNGMTIERVR VPLGVVGVIF ESRPNVAADA GVLCLKSGNA VILRGGSDSF RSCRAIHECL 190 200 210 220 230 240 VQGLREAGLP EAAITLVPTR DRAAVGMMLS GLNGAIDVIV PRGGKSLVAR VEQEARVPVF 250 260 270 280 290 300 AHLEGVNHVY VDASADLAMA KSIVLNAKMR RTGVCGAAET LLVDRAAAAT SLKPLVEMLI 310 320 330 340 350 360 EAGCEVRGDD VVQKTDARVK PANDDDWDTE YLDAIIAAKV VDGVDGAIAH IQNHGSHHTD 370 380 390 400 410 420 AIVSENEAAA KKFLSEVDSA IVLHNASTQF ADGGEFGFGA EIGIATGRFH ARGPVGAEQL 430 TSFKYRVHGT GQTRP

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC45694.1.
RefSeq	NP_767069.1.
3D structure databases
SMR	Q89X85. Positions 24-434.
ModBase	Search...
Genome annotation databases
GeneID	1054342.
GenomeReviews	Gene locus blr0429 in contig BA000040_GR.
KEGG	bja:blr0429.
NMPDR	fig|224911.1.peg.429.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
OMA	KTIVLNA.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR0429-MONOMER.
BRENDA	1.2.1.41. 280.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BRAJA

Accession

Primary (citable) accession number: Q89X85

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)